Comparative Evaluation of Two In Vitro Tests for Detection of Ivermectin Resistance in Haemonchus contortus of Small Ruminants in Uttar Pradesh, India

被引：1

作者：

Singh, Ekta ^{[1
,2
]}

Chandra, Dinesh ^{[1
]}

Prasad, Arvind ^{[1
]}

Kaur, Navneet ^{[1
]}

机构：

[1] ICAR Indian Vet Res Inst, Div Parasitol, Bareilly 243122, Uttar Pradesh, India

[2] CSK Himachal Pradesh Agr Univ, Dr GC Negi Coll Vet & Anim Sci, Dept Vet Parasitol, Palampur 176062, Himachal Prades, India

来源：

ACTA PARASITOLOGICA | 2021年 / 66卷 / 04期

关键词：

Ivermectin; Resistance; Haemonchus contortus; MALDT; LDA; LARVAL DEVELOPMENT TEST; ANTHELMINTIC RESISTANCE; NEMATODES;

D O I：

10.1007/s11686-021-00398-0

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Purpose Resistance against ivermectin in India has been detected solely implementing in vivo tests and there is no published report of resistance employing in vitro methods. The present study was carried out to study ivermectin resistance in H. contortus of small ruminants by two in vitro tests and to determine their efficacy in effectively detecting ivermectin resistance in the field isolates. Methods The present study was carried out in the small ruminant farms of the western region of Uttar Pradesh, India. Faecal samples with high egg per gram of faeces for H. contortus were selected. Two in vitro tests namely Micro agar larval development test and larval development assay were performed for assessing ivermectin resistance in field isolates of H. contortus by subjecting the eggs to different concentrations of ivermectin aglycone. Results The results of the two in vitro tests implicated towards the susceptibility of H. contortus isolates in the area under study. The standard deviations of the two tests was compared and was found to be statistically non-significant. Conclusion It can be said that both in vitro tests are reliable for detecting resistance against different anthelmintics including ivermectin.

引用

页码：1565 / 1569

页数：5

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