A new murine monoclonal antibody against Kx protein

被引：1
作者：
Hattab, C
Blanchard, D
Gane, P
Verkarre, V
Petit-Leroux, Y
Loirat, MJ
Cartron, JP
Bertrand, O
机构：
[1] Inst Natl Transfus Sanguine, INSERM, U76, F-75739 Paris 15, France
[2] ETS Loire Atlantique Vendee, Nantes, France
[3] Hop Necker Enfants Malad, Serv Anat Pathol, Paris, France
来源：
TRANSFUSION MEDICINE | 2003年 / 13卷 / 01期
关键词：
Kell; Kx; monoclonal antibody; membrane proteins;
D O I：
10.1046/j.1365-3148.2003.00414.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Mice immunized with a synthetic peptide located on an intracellular segment of the polytopic Kx protein (37 kDa) from human red blood cells (RBCs) produced a monoclonal antibody called C7B8. As expected, this antibody did not agglutinate common RBCs but reacted with permeabilized cells in flow cytometry. C7B8 recognizes the Kx protein on Western blots. Cross-reactivity of C7B8 with human calpain of human muscle extracts was demonstrated by Western blot analysis. This cross-reactivity precludes the use of C7B8 for Kx tissue distribution studies, but immobilized C7B8 was a convenient tool for purification of the Kell-Kx complex from RBC membrane extract by immunochromatography.
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收藏
页码：43 / 48
页数：6
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