TI-VAMP/VAMP7 and VAMP3/cellubrevin: two v-SNARE proteins involved in specific steps of the autophagy/multivesicular body pathways

被引:384
作者
Marcelo Fader, Claudio [1 ]
German Sanchez, Diego [1 ]
Belen Mestre, Maria [1 ]
Colombo, Maria Isabel [1 ]
机构
[1] Univ Nacl Cuyo, CONICET, Fac Ciencias Med,Ctr Univ, Inst Histol & Embriol,Lab Biol Celular & Mol, RA-5500 Mendoza, Argentina
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2009年 / 1793卷 / 12期
关键词
SNAREs; VAMP7; VAMP3; Multivesicular bodies; Autophagy; Autophagosome; Exosomes; LC3; Rab11; K562; TOXIN-MEDIATED CLEAVAGE; RETICULOCYTE MATURATION; PLASMA-MEMBRANE; TRANSFERRIN RECEPTOR; IN-VITRO; MULTIVESICULAR BODIES; AUTOPHAGIC VACUOLES; VESICULAR TRANSPORT; TETANUS TOXIN; RAT RETICULOCYTES;
D O I
10.1016/j.bbamcr.2009.09.011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
During reticulocyte maturation, some membrane proteins and organelles that are not required in the mature red cell are lost. Several of these proteins are released into the extracellular medium associated with the internal vesicles present in multivesicular bodies (MVBs). Likewise, organelles such as mitochondria and endoplasmic reticulum are wrapped into double membrane vacuoles (i.e., autophagosomes) and degraded via autophagy. Morphological, molecular, and biochemical studies have shown that autophagosomes fuse with MVBs forming the so-called amphisomes, a prelysosomal hybrid organelle. SNAREs are key molecules of the vesicle fusion machinery. TI-VAMP/VAMP7 and VAMP3/cellubrevin are two v-SNARE proteins involved in the endocytic and exocytic pathways. We have previously shown that in the human leukemic K562 cells, Rab11 decorates MVBs and it is necessary for fusion between autophagosomes with MVBs. In the present report, we present evidence indicating that VAMP3 is required for the fusion between MVBs with autophagosomes to generate the amphisome, allowing the maturation of the autophagosome, but it does not seem to be involved in the next step, i.e., fusion with the lysosome. On the other hand, we demonstrate that VAMP7 is necessary for this latter event, allowing the completion of the autophagic pathway. Furthermore, VAMP7 and ATPase NSF, a protein required for SNAREs disassembly, participate in the fusion between MVBs with the plasma membrane to release the internal vesicles (i.e., exosomes) into the extracellular medium. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:1901 / 1916
页数:16
相关论文
共 88 条
[1]   VAMP-7 mediates vesicular transport from endosomes to lysosomes [J].
Advani, RJ ;
Yang, B ;
Prekeris, R ;
Lee, KC ;
Klumperman, J ;
Scheller, RH .
JOURNAL OF CELL BIOLOGY, 1999, 146 (04) :765-775
[2]   Seven novel mammalian SNARE proteins localize to distinct membrane compartments [J].
Advani, RJ ;
Bae, HR ;
Bock, JB ;
Chao, DS ;
Doung, YC ;
Prekeris, R ;
Yoo, JS ;
Scheller, RH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (17) :10317-10324
[3]   Cross talk between tetanus neurotoxin-insensitive vesicle-associated membrane protein-mediated transport and L1-mediated adhesion [J].
Alberts, P ;
Rudge, R ;
Hinners, I ;
Muzerelle, A ;
Martinez-Arca, S ;
Irinopoulou, T ;
Marthiens, V ;
Tooze, S ;
Rathjen, F ;
Gaspar, P ;
Galli, T .
MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (10) :4207-4220
[4]   Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs [J].
Antonin, W ;
Fasshauer, D ;
Becker, S ;
Jahn, R ;
Schneider, TR .
NATURE STRUCTURAL BIOLOGY, 2002, 9 (02) :107-111
[5]   Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body [J].
Babst, M ;
Katzmann, DJ ;
Snyder, WB ;
Wendland, B ;
Emr, SD .
DEVELOPMENTAL CELL, 2002, 3 (02) :283-289
[6]   A protein's final ESCRT [J].
Babst, M .
TRAFFIC, 2005, 6 (01) :2-9
[7]   ESCRT-III: An endosome-associated heterooligomeric protein complex required for MVB sorting [J].
Babst, M ;
Katzmann, DJ ;
Estepa-Sabal, EJ ;
Meerloo, T ;
Emr, SD .
DEVELOPMENTAL CELL, 2002, 3 (02) :271-282
[8]   Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes [J].
Bache, KG ;
Brech, A ;
Mehlum, A ;
Stenmark, H .
JOURNAL OF CELL BIOLOGY, 2003, 162 (03) :435-442
[9]   Isolation and characterization of rat liver amphisomes - Evidence for fusion of autophagosomes with both early and late endosomes [J].
Berg, TO ;
Fengsrud, M ;
Stromhaug, PE ;
Berg, T ;
Seglen, PO .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (34) :21883-21892
[10]  
BIEDERBICK A, 1995, EUR J CELL BIOL, V66, P3