Electrochemical detection of DNA hybridization based on DNA-templated assembly of silver cluster

被引：76

作者：

Wang, J ^{[1
]}

Rincón, O

Polsky, R

Dominguez, E

机构：

[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA

[2] Univ Alcala de Henares, Dept Analyt Chem & Chem Engn, Madrid 28871, Spain

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2003年 / 5卷 / 01期

基金：

美国国家科学基金会;

关键词：

DNA detection; silver; stripping analysis; DNA metallization;

D O I：

10.1016/S1388-2481(02)00542-8

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

The growth of metals on DNA templates has generated considerable interest in connection to the design of metallic nanostructures. Here we exploit the DNA-induced generation of metal clusters for developing an electrical biosensing protocol. The new hybridization assay employs a probe-modified gold surface, and is based on the electrostatic 'collection' of silver cations along the DNA duplex, the reductive formation of silver nanoclusters along the DNA backbone, dissolution of the silver aggregate and stripping potentiometric detection of the dissolved silver at a thick-film carbon electrode. The new protocol thus combines the inherent signal amplification of stripping analysis with effective discrimination against nonhybridized DNA. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：83 / 86

页数：4

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