Visualizing voltage dynamics in zebrafish heart

被引：53
作者：
Tsutsui, Hidekazu [1 ,2 ,3 ]
Higashijima, Shin-ichi [4 ]
Miyawaki, Atsushi [2 ]
Okamura, Yasushi [1 ]
机构：
[1] Osaka Univ, Grad Sch Med, Dept Physiol, Lab Integrat Physiol, Suita, Osaka 5650871, Japan
[2] RIKEN, Brain Sci Inst, Lab Cell Funct Dynam, Wako, Saitama 3510198, Japan
[3] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3510198, Japan
[4] Natl Inst Nat Sci, Okazaki Inst Integrat Biosci, Natl Inst Physiol Sci, Okazaki, Aichi 4448787, Japan
来源：
JOURNAL OF PHYSIOLOGY-LONDON | 2010年 / 588卷 / 12期
关键词：
DRUGS; HERG;
D O I：
10.1113/jphysiol.2010.189126
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The zebrafish heart provides a useful vertebrate cardiovascular model with outstanding advantages, including genetic manipulatability, optical accessibility and rapid development. In addition, an emerging topic in cardiotoxicity assay and drug discovery is its use in phenotype-based chemical screening. Here, we report a technique that allows non-invasive voltage mapping in beating heart using a genetically encoded probe for transmembrane potential. Application of the anti-allergy compound astemizole resulted in aberrant propagation of excitation, which accounted for a lack of ventricular contraction. This optical method will provide new opportunities in broad areas of physiological, developmental and pharmacological cardiovascular research.
引用
收藏
页码：2017 / 2021
页数：5
相关论文
共 13 条
[1]   Genetically encoded fluorescent sensors of membrane potential [J].
Baker, B. J. ;
Mutoh, H. ;
Dimitrov, D. ;
Akemann, W. ;
Perron, A. ;
Iwamoto, Y. ;
Jin, L. ;
Cohen, L. B. ;
Isacoff, E. Y. ;
Pieribone, V. A. ;
Hughes, T. ;
Knopfel, T. .
BRAIN CELL BIOLOGY, 2008, 36 (1-4) :53-67
[2]   High-throughput assay for small molecules that modulate zebrafish embryonic heart rate [J].
Burns, CG ;
Milan, DJ ;
Grande, EJ ;
Rottbauer, W ;
MacRae, CA ;
Fishman, MC .
NATURE CHEMICAL BIOLOGY, 2005, 1 (05) :263-264
[3]   Reporting ethical matters in The Journal of Physiology: standards and advice [J].
Drummond, Gordon B. .
JOURNAL OF PHYSIOLOGY-LONDON, 2009, 587 (04) :713-719
[4]   Temperature acclimation modifies sinoatrial pacemaker mechanism of the rainbow trout heart [J].
Haverinen, Jaakko ;
Vornanen, Matti .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2007, 292 (02) :R1023-R1032
[5]   Germ-line transmission of a myocardium-specific GFP transgene reveals critical regulatory elements in the cardiac myosin light chain 2 promoter of zebrafish [J].
Huang, CJ ;
Tu, CT ;
Hsiao, CD ;
Hsieh, FJ ;
Tsai, HJ .
DEVELOPMENTAL DYNAMICS, 2003, 228 (01) :30-40
[6]   Zebrafish embryos express an orthologue of HERG and are sensitive toward a range and are of QT-prolonging drugs inducing severe arrhythmia [J].
Langheinrich, U ;
Vacun, G ;
Wagner, T .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2003, 193 (03) :370-382
[7]   Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development [J].
Liebling, Michael ;
Forouhar, Arian S. ;
Wolleschensky, Ralf ;
Zimmermann, Bernhard ;
Ankerhold, Richard ;
Fraser, Scott E. ;
Gharib, Morteza ;
Dickinson, Mary E. .
DEVELOPMENTAL DYNAMICS, 2006, 235 (11) :2940-2948
[8]   In vivo recording of adult zebrafish electrocardiogram and assessment of drug-induced QT prolongation [J].
Milan, David J. ;
Jones, Ian L. ;
Ellinor, Patrick T. ;
MacRae, Calum A. .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2006, 291 (01) :H269-H273
[9]   Drugs that induce repolarization abnormalities cause bradycardia in zebrafish [J].
Milan, DJ ;
Peterson, TA ;
Ruskin, JN ;
Peterson, RT ;
MacRae, CA .
CIRCULATION, 2003, 107 (10) :1355-1358
[10]   Functional and morphological evidence for a ventricular conduction system in zebrafish and Xenopus hearts [J].
Sedmera, D ;
Reckova, M ;
deAlmeida, A ;
Sedmerova, M ;
Biermann, M ;
Volejnik, J ;
Sarre, A ;
Raddatz, E ;
McCarthy, RA ;
Gourdie, RG ;
Thompson, RP .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2003, 284 (04) :H1152-H1160
12