The permeability effect of microcystin-RR on Escherichia coli and Bacillus subtilis

被引：6
作者：
Yang CuiYun [2 ,3 ]
Xia ChuanHai [2 ]
Zhou ShiWei [2 ]
Liu YongDing [1 ]
机构：
[1] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
[2] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
[3] Shandong Key Lab Ecoenvironm Sci Yellow River Del, Binzhou 256600, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2010年 / 55卷 / 18期
关键词：
MC-RR; Escherichia coli; Bacillus subtilis; permeability; protein; carbonhydrate; LYMPHOCYTES IN-VITRO; ANTIOXIDANT SYSTEM; CYANOBACTERIA; GROWTH; LR; EXTRACTS; AERUGINOSA; APOPTOSIS; SPAIN; BASIN;
D O I：
10.1007/s11434-010-3169-5
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Microcystins are a kind of cyclic hepatoxins produced by many species of cyanobacteria. Most previous work have been done on the toxic effects of microcystins on animals and plants. However, the reports about the effect of microcystins on microbial cells are very limited. In this work, the permeability of MC-RR on the cell outer membrane of Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) was discussed. The permeability effect of MC-RR on the cell outer membrane of E. coli and B. subtilis under different concentrations was demonstrated by a rapid and sustained reduction in the A(675) values of lysozyme-treated cells. The decrease of the absorbance values showed a time-and dose-effect. The extravasations of protein and carbonhydrate increased with the increment of the treated-concentration of MC-RR. The results showed that MC-RR could increase the permeability of cell outer membranes of E. coli and B. subtilis. The synergistic effects of MC-RR and lysozyme on bacteria indicated that MC-RR might play an ecological role in bacteria in combination with other substances in some aquatic environments.
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页码：1894 / 1898
页数：5
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