The inhibitory impacts of Lactobacillus rhamnosus GG-derived extracellular vesicles on the growth of hepatic cancer cells

被引：111
作者：
Behzadi, Elham [1 ]
Hosseini, Hamideh Mahmoodzadeh [1 ]
Fooladi, Abbas Ali Imani [1 ]
机构：
[1] Baqiyatallah Univ Med Sci, Appl Microbiol Res Ctr, Vanak Sq Mollasadra St,POB 19395-5487, Tehran, Iran
来源：
MICROBIAL PATHOGENESIS | 2017年 / 110卷
关键词：
Lactobacillus rhamnosus GG; Extracellular vesicles; Hepatic cancer; Apoptosis; Probiotic; GRAM-POSITIVE BACTERIA; MEMBRANE-VESICLES; APOPTOSIS; DEATH;
D O I：
10.1016/j.micpath.2017.06.016
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Bacterial extracellular vesicles (EVs) have come forth into notice as possible important agent to mediate host-pathogen interactions. In this scientific research, the authors have tried to find out the effect of EVs derived from Lactobacillus rhamnosus GG (LDEVs) on the apoptosis induction in HepG2 cell line. The EVs were purified from the conditioned medium of Lactobacillus rhamnosus GG using ultrafiltration and confirmed by transmission electron microscopy (TEM). The HepG2 cells were treated with different concentrations of purified LDEVs and the cytotoxicity and their effects on the expression of bcl-2 and box genes were assessed by the MIT assay and semi-quantitative RT-PCR, respectively. The MIT assay showed that only 100 mu g/m1 of LDEVs had a significant cytotoxic effect on cancer cells (p < 0.05). The apoptotic index (bax/bcl2 expression ratio) was significantly increased after treating with 50 and 100 mu g/ml LDEVs (p < 0.05). Increased bax/bcl-2 ratio was led to cancer cell death. (C) 2017 Elsevier Ltd. All rights reserved.
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页码：1 / 6
页数：6
相关论文
共 40 条
[1]  
Alves NJ, 2015, THER DELIV, V6, P873, DOI [10.4155/TDE.15.40, 10.4155/tde.15.40]
[2]  
[Anonymous], 2012, J BASIC MICROBIOL, DOI [10.1002/jobm.201100290, DOI 10.1002/JOBM.201100290]
[3]   Therapeutic potential of pterostilbene against pancreatic beta-cell apoptosis mediated through Nrf2 [J].
Bhakkiyalakshmi, Elango ;
Shalini, Devibalan ;
Sekar, Thillai Veerapazham ;
Rajaguru, Palanisamy ;
Paulmurugan, Ramasamy ;
Ramkumar, Kunka Mohanram .
BRITISH JOURNAL OF PHARMACOLOGY, 2014, 171 (07) :1747-1757
[4]   Parasporin-2 from a New Bacillus thuringiensis 4R2 Strain Induces Caspases Activation and Apoptosis in Human Cancer Cells [J].
Brasseur, Kevin ;
Auger, Pascal ;
Asselin, Eric ;
Parent, Sophie ;
Cote, Jean-Charles ;
Sirois, Marc .
PLOS ONE, 2015, 10 (08)
[5]   Through the wall: extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi [J].
Brown, Lisa ;
Wolf, Julie M. ;
Prados-Rosales, Rafael ;
Casadevall, Arturo .
NATURE REVIEWS MICROBIOLOGY, 2015, 13 (10) :620-630
[6]   Oral administration of a probiotic Lactobacillus modulates cytokine production and TLR expression improving the immune response against Salmonella enterica serovar Typhimurium infection in mice [J].
Castillo, Natalia A. ;
Perdigon, Gabriela ;
de Moreno de LeBlanc, Alejandra .
BMC MICROBIOLOGY, 2011, 11
[7]   Exosomes derived from SW480 colorectal cancer cells promote cell migration in HepG2 hepatocellular cancer cells via the mitogen-activated protein kinase pathway [J].
Chiba, Mitsuru ;
Watanabe, Narumi ;
Watanabe, Miki ;
Sakamoto, Maki ;
Sato, Akika ;
Fujisaki, Mizuki ;
Kubota, Shiori ;
Monzen, Satoru ;
Maruyama, Atsushi ;
Nanashima, Naoki ;
Kashiwakura, Ikuo ;
Nakamura, Toshiya .
INTERNATIONAL JOURNAL OF ONCOLOGY, 2016, 48 (01) :305-312
[8]   The potential mechanisms involved in the anti-carcinogenic action of probiotics [J].
Commane, D ;
Hughes, R ;
Shortt, C ;
Rowland, I .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2005, 591 (1-2) :276-289
[9]   Apoptosis: A review of programmed cell death [J].
Elmore, Susan .
TOXICOLOGIC PATHOLOGY, 2007, 35 (04) :495-516
[10]   Staphylococcal enterotoxin B/texosomes as a candidate for breast cancer immunotherapy [J].
Fooladi, Abbas Ali Imani ;
Halabian, Raheleh ;
Mahdavi, Mehdi ;
Amin, Mohsen ;
Hosseini, Hamideh Mahmoodzadeh .
TUMOR BIOLOGY, 2016, 37 (01) :739-748
1234