A Label-free Electrochemical Biosensor for Acrylamide Based on DNA Immobilized on Graphene Oxide-Modified Glassy Carbon Electrode

被引：0

作者：

Li, Dan ^{[1
]}

Xu, Yunmei ^{[1
]}

Zhang, Ling ^{[1
]}

Tong, Haixia ^{[1
]}

机构：

[1] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect & Transporta, Changsha 410114, Hunan, Peoples R China

来源：

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE | 2014年 / 9卷 / 12期

关键词：

Acrylamide; DNA; Graphene oxide; Differential pulse voltammetry; Electrochemical biosensor; NANOTUBES; FLUORESCENCE; ADSORPTION; EXPOSURE;

D O I：

暂无

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Acrylamide (AA) is a widely used chemical compound, which has strong neurotoxicity, and potential carcinogenicity and genotoxicity. In the present work, the interaction between AA and DNA was confirmed by UV-visible spectroscopy. According to such an interaction, a label-free DNA biosensor for electrochemical determination of AA was proposed. To fabricate the sensor, graphene oxide (GO) was firstly coated on glassy carbon electrode (GCE) surface. Then DNA was immobilized on GO/GCE by electroadsorption. Due to large surface area of GO, DNA was effectively immobilized on the electrode surface. Moreover, the unique nanostructure and excellent electron transfer ability of GO significantly promoted the direct electron transfer of DNA. Thus, DNA showed two strong oxidation peaks on GO/GCE, which could be used as the electrochemical signal for AA sensing. The Influences of adsorption potential and adsorption time on the immobilized DNA and pH for AA sensing were systematically investigated. Under optimum conditions, the response of DNA/GO/GCE was linear to the concentration of AA from 5.0x10(-8) to 1.0x10(-3) mol/L. Moreover, the sensor showed good reproducibility and high stability.

引用

页码：7217 / 7227

页数：11

共 25 条

[1] Wavelength Dependent UV Inactivation and DNA Damage of Adenovirus as Measured by Cell Culture Infectivity and Long Range Quantitative PCR [J].

Beck, Sara E. ;

Rodriguez, Roberto A. ;

Linden, Karl G. ;

Hargy, Thomas M. ;

Larason, Thomas C. ;

Wright, Harold B. .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2014, 48 (01) :591-598

[2] Multi-walled carbon nanotubes modified glassy carbon electrode for sensitive determination of ketoconazole [J].

Borowiec, Joanna ;

Wei, Lili ;

Zhu, Lihua ;

Zhang, Jingdong .

ANALYTICAL METHODS, 2012, 4 (02) :444-448

[3] Electron transfer in DNA and in DNA-related biological processes. Electrochemical insights [J].

Boussicault, Fabien ;

Robert, Marc .

CHEMICAL REVIEWS, 2008, 108 (07) :2622-2645

[4] Effect of pH and applied potential on the adsorption of DNA on highly oriented pyrolytic graphite electrodes. Atomic force microscopy surface characterisation [J].

Brett, AMO ;

Chiorcea, AM .

ELECTROCHEMISTRY COMMUNICATIONS, 2003, 5 (02) :178-183

[5] Effects of acrylamide exposure on serum hormones, gene expression, cell proliferation, and histopathology in male reproductive tissues of Fischer 344 rats [J].

Camacho, L. ;

Latendresse, J. R. ;

Muskhelishvili, L. ;

Patton, R. ;

Bowyer, J. F. ;

Thomas, M. ;

Doerge, D. R. .

TOXICOLOGY LETTERS, 2012, 211 (02) :135-143

[6] Acrylamide and 5-hydroxymethylfurfural (HMF): A review on metabolism, toxicity, occurrence in food and mitigation strategies [J].

Capuano, Edoardo ;

Fogliano, Vincenzo .

LWT-FOOD SCIENCE AND TECHNOLOGY, 2011, 44 (04) :793-810

[7]

Chen JY, 2013, INT J ELECTROCHEM SC, V8, P3963

[8] Acrylamide formation in traditional Czech leavened wheat-rye breads and wheat rolls [J].

Forstova, Veronika ;

Belkova, Beverly ;

Riddellova, Katerina ;

Vaclavik, Lukas ;

Prihoda, Josef ;

Hajslova, Jana .

FOOD CONTROL, 2014, 38 :221-226

[9] Adsorption and Electrooxidation of DNA at Glassy Carbon Electrodes Modified with Multiwall Carbon Nanotubes Dispersed in Glucose Oxidase [J].

Gutierrez, Fabiana ;

Rubianes, Maria D. ;

Rivas, Gustavo A. .

ELECTROANALYSIS, 2013, 25 (05) :1135-1142

[10] Effect of standard phenolic compounds and olive oil phenolic extracts on acrylamide formation in an emulsion system [J].

Kotsiou, Kali ;

Tasioula-Margari, Maria ;

Capuano, Edoardo ;

Fogliano, Vincenzo .

FOOD CHEMISTRY, 2011, 124 (01) :242-247

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