Early maternal deprivation impairs learning and memory and alters hippocampal gene expression in adult male rats

被引：5

作者：

Xiong, Xiaofan ^{[1
,3
]}

Han, Lin ^{[1
,2
]}

Fan, Meiyang ^{[1
]}

Zhang, Lingyu ^{[1
]}

Liu, Liying ^{[1
,2
]}

Guo, Chen ^{[1
]}

Wu, Qiuhua ^{[1
]}

Wang, Xiaofei ^{[2
]}

Sun, Ruifang ^{[2
,4
]}

Ni, Lei ^{[1
]}

Huang, Chen ^{[1
,2
]}

Yang, Juan ^{[1
,2
]}

机构：

[1] Xi An Jiao Tong Univ, Sch Basic Med Sci, Dept Cell Biol & Genet, Hlth Sci Ctr, Xian 710061, Peoples R China

[2] Xi An Jiao Tong Univ, Key Lab Environm & Genes Related Dis, Minist Educ China, Xian 710061, Peoples R China

[3] Xi An Jiao Tong Univ, Natl Local Joint Engn Res Ctr Biodiagnost & Bioth, Affiliated Hosp 2, Xian 710061, Peoples R China

[4] Xi An Jiao Tong Univ, Sch Basic Med Sci, Dept Pathol, Hlth Sci Ctr, Xian 710061, Peoples R China

来源：

NEUROBIOLOGY OF LEARNING AND MEMORY | 2021年 / 183卷

基金：

美国国家科学基金会;

关键词：

Mother deprivation; Learning and memory; Hippocampus; Proteins; ALPHA-II SPECTRIN; ATP-CITRATE LYASE; SEPARATION; STRESS; MODEL;

D O I：

10.1016/j.nlm.2021.107479

中图分类号：

B84 [心理学]; C [社会科学总论]; Q98 [人类学];

学科分类号：

03 ; 0303 ; 030303 ; 04 ; 0402 ;

摘要：

Maternal deprivation (MD) in early life severely disrupts hippocampal development, leading to persistent cognitive and behavior deficits. The current study uncovered that early MD (P1-P21) impaired spatial learning and memory capacity detected by Morris water maze (MWM) tests from juvenile (P31) to adult (P81) rats compared to age-matched controls. And the protein expression in hippocampus were detected by twodimensional gel electrophoresis (2-DE) before MWM, respectively. Protein changes in hippocampal were examined to identify the molecular pathways underlying MD-induced hippocampal dysfunction. There were 11 differentially expressed proteins analyzed between adult MD and control male rats, while the 8 proteins were then identified by UPLC-ESI-Q-TOF-MS. Gene Ontology (GO) annotations of the identified proteins were related to neuronal and glial cytoskeletal dynamics, membrane signaling, stress responses, biosynthesis, and metabolism. The different expression proteins spectrin alpha chain, non-erythrocytic 1 (Sptan1), ATP-citrate synthase (Acly), and heat shock protein 90-alpha (Hsp90aa1) have been verified by western blot analysis, and their expression levels showed consistent with 2-DE analysis. In addition, glial fibrillary acidic protein (GFAP) was also found reduced in adult hippocampus of MD rats. This study identifies candidate proteins encompassing a range of functional categories that may contribute to persistent learning and memory deficits due to early life MD.

引用

页数：8

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