Metabolomic approach for characterization of phenolic compounds in different wheat genotypes during grain development

被引:72
作者
Barros Santos, Millena Cristina [1 ]
da Silva Lima, Luciana Ribeiro [1 ,2 ]
Nascimento, Fabiana Ramos [1 ]
do Nascimento, Talita Pimenta [1 ]
Cameron, Luiz Claudio [3 ]
Larraz Ferreira, Mariana Simoes [1 ,2 ,3 ]
机构
[1] Fed Univ State Rio de Janeiro, UNIRIO, Food & Nutr Grad P Twain, Lab Bioactives, Av Pasteur 296, BR-22290240 Urca, RJ, Brazil
[2] UNIRIO, Nutr Sch, Dept Food Sci, Urca, RJ, Brazil
[3] UNIRIO, Ctr Innovat Mass Spectrometry, Lab Prot Biochem, Urca, RJ, Brazil
关键词
UPLC-MS; Triticum aestivum; Bioactive compounds; Free phenolics; Bound phenolics; Folin-Ciocalteu; ANTIOXIDANT PROPERTIES; ACID PROFILES; BRAN; BIOSYNTHESIS; DIVERSITY; VARIETIES; PRODUCTS; IMMATURE; CEREALS; BREAD;
D O I
10.1016/j.foodres.2018.08.034
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The phenolic-profiling of seven different wheat (Triacton aestivum) genotypes was investigated for the first time during different stages of grain development (milky, softy, physiological maturity and mature). Free and bound phenolic compounds were extracted separately and analyzed by UPLC-QTOF-MSE. Total phenolic content significantly decreased, up to 50% depending on the genotype, towards the maturation of grain. The highest content (free and bound) was observed in the most immature grains, while the lowest level was found in mature grains (408.0 and 165.0 GAE mg/100 g, respectively). Globally, 237 phenolic compounds were identified, divided into 5 classes: flavonoids (85), phenolic acids (77), other polyphenols (51), lignans (16) and stilbenes (8). UPLC-MS results showed a progressively decrease of the number of phenolic identification (ID) all along grain development, milky (213), softy (192), physiological maturity (169) and mature (144). The proportion bound to free phenolic progressively increased, reaching the maximum at physiological maturity, indicating a possible enzymatic reactions and complexation during grain growth. Ferulic acid, diphyllin, 4-hydroxybenzoic acid, ferulic acid isomer, apigenin 7-O-apiosyl-glucoside isomer and myricetin isomer were the most abundant compounds. Chemometric tools showed a clear separation between immature and mature grain for all genotypes. Phenolic profile varied significantly among genotypes, this result can help the selection of varieties towards a higher retention of bioactive compounds. Noteworthy, immature wheat grains can be considered a rich source of phenolic compounds and as an attractive ingredient to incorporate to functional foods.
引用
收藏
页码:118 / 128
页数:11
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