Interactions of the β-blocker drug, propranolol, with detergents, β-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging

被引：13

作者：

Bisby, R. H. ^{[1
]}

Botchway, S. W. ^{[2
]}

Crisostomo, A. G. ^{[1
]}

Karolin, J. ^{[3
]}

Parker, A. W. ^{[2
]}

Schroeder, L. ^{[1
]}

机构：

[1] Univ Salford, Sch Environm & Life Sci, Salford M5 4WT, Lancs, England

[2] Rutherford Appleton Lab, Lasers Sci Facil, Cent Laser Facil, Sci & Technol Facil Council, Didcot OX11 0QX, Oxon, England

[3] Univ Strathclyde, SUPA, Dept Phys, Glasgow, Lanark, Scotland

来源：

SPECTROSCOPY-AN INTERNATIONAL JOURNAL | 2010年 / 24卷 / 1-2期

关键词：

Propranolol; fluorescence; lifetime; quenching; micelle; cyclodextrin; imaging; live cell; BINDING; WATER;

D O I：

10.1155/2010/129574

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Interactions of the beta-blocker drug, propranolol, with amphipathic systems have been studied using fluorescence spectroscopy. The results show a strong binding of propranolol with micelles of sodium dodecyl sulfate revealed through changes in the fluorescence spectrum and an increase in fluorescence lifetime. Quenching of propranolol fluorescence by iodide is used to demonstrate interaction with beta-cyclodextrin. At high concentrations, self-quenching of propranolol fluorescence was also observed with kappa(q), = 2.5 x 10(9) dm(3) mol(-1) s(-1). Two-photon excited (630 nm) fluorescence lifetime imaging of propranolol in cells showed propranolol to be widely distributed in the cell cytoplasm, with fluorescence lifetimes shorter than in solution. The results suggest that intracellular propranolol is mainly confined within the aqueous cytoplasm and rather than membrane associated.

引用

页码：137 / 142

页数：6

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