Genome-Wide Identification of Cotton (Gossypium spp.) Trehalose-6-Phosphate Phosphatase (TPP) Gene Family Members and the Role of GhTPP22 in the Response to Drought Stress

被引：21
作者：
Wang, Weipeng [1 ,2 ]
Cui, Hua [3 ]
Xiao, Xiangfen [4 ]
Wu, Bingjie [2 ]
Sun, Jialiang [2 ]
Zhang, Yaxin [2 ]
Yang, Qiuyue [2 ]
Zhao, Yuping [2 ]
Liu, Guoxiang [1 ]
Qin, Tengfei [1 ,2 ,5 ]
机构：
[1] Chinese Acad Agr Sci, Key Lab Tobacco Improvement & Biotechnol, Tobacco Res Inst, Qingdao 266100, Peoples R China
[2] Liaocheng Univ, Coll Agr, Liaocheng 252059, Shandong, Peoples R China
[3] Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Key Lab Cell & Gene Circuit Design, Shenzhen 518055, Peoples R China
[4] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 201602, Peoples R China
[5] Beijing Capital Agribusiness Future Biotechnol, Beijing 100088, Peoples R China
来源：
PLANTS-BASEL | 2022年 / 11卷 / 08期
关键词：
cotton; trehalose-6-phosphate phosphatase (TPP); gene expression; drought stress; trehalose 6-phosphate (T6P); TREHALOSE; 6-PHOSPHATE; ARABIDOPSIS-THALIANA; TOLERANCE; INHIBITION; EXPRESSION; EVOLUTION; OVEREXPRESSION; ACCUMULATION; ARCHITECTURE; DATABASE;
D O I：
10.3390/plants11081079
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Trehalose-6-phosphate phosphatase (TPP) is a key enzyme involved in trehalose synthesis in higher plants. Previous studies have shown that TPP family genes increase yields without affecting plant growth under drought conditions, but their functions in cotton have not been reported. In this study, 17, 12, 26 and 24 TPP family genes were identified in Gossypium arboreum, Gossypium raimondii, Gossypium barbadense and Gossypium hirsutum, respectively. The 79 TPP family genes were divided into three subgroups by phylogenetic analysis. Virus-induced gene silencing (VIGS) of GhTPP22 produced TRV::GhTPP22 plants that were more sensitive to drought stress than the control plants, and the relative expression of GhTPP22 was decreased, as shown by qRT-PCR. Moreover, we analysed the gene structure, targeted small RNAs, and gene expression patterns of TPP family members and the physicochemical properties of their encoded proteins. Overall, members of the TPP gene family in cotton were systematically identified, and the function of GhTPP22 under drought stress conditions was preliminarily verified. These findings provide new information for improving drought resistance for cotton breeding in the future.
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页数：18
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