Pseudoalteromonas aestuariivivens sp nov., isolated from a tidal flat

A Gram-stain-negative, aerobic, motile, rod-shaped or ovoid bacterial strain, designated DB-2(T), was isolated from a tidal flat of the Yellow Sea in South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain DB-2(T) grew optimally at 30 degrees C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain DB-2(T) belonged to the genus Pseudoalteromonas. Strain DB-2(T) exhibited 16S rRNA gene sequence similarities of 97.17-97.36 % to the type strains of Pseudoalteromonas mariniglutinosa, Pseudoalteromonas spongiae and Pseudoalteromonas tetraodonis and of 93.79-96.99 % to the type strains of the other species of the genus Pseudoalteromonas. Strain DB-2(T) contained Q-8 as the predominant ubiquinone and C-16 : 0, C-18 : 1 omega 7c, summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) and C-12 : 0 3-OH as the major fatty acids. The major polar lipids detected in strain DB-2(T) were phosphatidylglycerol, phosphatidylethanolamine, two unidentified glycolipids, an unidentified phospholipid and an unidentified aminolipid. The DNA G+C content of strain DB-2(T) was 54.9 +/- 0.2 mol% and mean DNA-DNA relatedness values with the type strains of P. mariniglutinosa, P. spongiae and P. tetraodonis were 10-17 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain DB-2(T) is separated from recognized species of the genus Pseudoalteromonas. On the basis of these data, strain DB-2(T) is considered to represent a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas aestuariivivens sp. nov. is proposed. The type strain is DB-2(T) (=KCTC 42779(T) =CECT 8945(T)).