Glutathionyl-hydroquinone reductases from poplar are plastidial proteins that deglutathionylate both reduced and oxidized glutathionylated quinones

被引：18

作者：

Lallement, Pierre-Alexandre ^{[1
,2
]}

Meux, Edgar ^{[1
,2
]}

Gualberto, Jose M. ^{[3
]}

Dumarcay, Stephane ^{[4
]}

Favier, Frederique ^{[5
,6
]}

Didierjean, Claude ^{[5
,6
]}

Saul, Frederick ^{[7
]}

Haouz, Ahmed ^{[7
]}

Morel-Rouhier, Melanie ^{[1
,2
]}

Gelhaye, Eric ^{[1
,2
]}

Rouhier, Nicolas ^{[1
,2
]}

Hecker, Arnaud ^{[1
,2
]}

机构：

[1] Univ Lorraine, UMR1136, F-54500 Vandoeuvre Les Nancy, France

[2] INRA, UMR1136, F-54280 Champenoux, France

[3] CNRS, Inst Biol Mol Plantes, UPR 2357, F-67084 Strasbourg, France

[4] Lab Etud & Rech Mat Bois, EA 1093, F-54506 Vandoeuvre Les Nancy, France

[5] Univ Lorraine, Fac Sci & Technol, CRM2, UMR 7036, F-54506 Vandoeuvre Les Nancy, France

[6] CNRS, CRM2, Fac Sci & Technol, UMR 7036, F-54506 Vandoeuvre Les Nancy, France

[7] CNRS, Inst Pasteur, UMR 3528, F-75724 Paris, France

来源：

FEBS LETTERS | 2015年 / 589卷 / 01期

关键词：

Deglutathionylation; Glutathione transferase; Plastid; Poplar; S-GLUTATHIONYL-(CHLORO)HYDROQUINONE REDUCTASES; FUNCTIONAL DIVERGENCE; ARABIDOPSIS-THALIANA; TRANSFERASES; PLANTS; IDENTIFICATION; MECHANISM; GENE; INHIBITION; METABOLISM;

D O I：

10.1016/j.febslet.2014.11.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutathionyl-hydroquinone reductases (GHRs) catalyze the deglutathionylation of quinones via a catalytic cysteine. The two GHR genes in the Populus trichocarpa genome, Pt-GHR1 and Pt-GHR2, are primarily expressed in reproductive organs. Both proteins are localized in plastids. More specifically, Pt-GHR2 localizes in nucleoids. At the structural level, Pt-GHR1 adopts a typical GHR fold, with a dimerization interface comparable to that of the bacterial and fungal GHR counterparts. Pt-GHR1 catalyzes the deglutathionylation of both reduced and oxidized glutathionylated quinones, but the enzyme is more catalytically efficient with the reduced forms. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：37 / 44

页数：8

共 28 条

[11]

Jacquot JP, 2013, OXIDATIVE STRESS RED, P213, DOI 10.1007/978-94-007-5787-5_8

[12]

Kim J., 2014, CRYSTAL STRUCTURE GL

[13] The still mysterious roles of cysteine-containing glutathione transferases in plants [J].

Lallement, Pierre-Alexandre ;

Brouwer, Bastiaan ;

Keech, Olivier ;

Hecker, Arnaud ;

Rouhier, Nicolas .

FRONTIERS IN PHARMACOLOGY, 2014, 5

[14] Structural and enzymatic insights into Lambda glutathione transferases from Populus trichocarpa, monomeric enzymes constituting an early divergent class specific to terrestrial plants [J].

Lallement, Pierre-Alexandre ;

Meux, Edgar ;

Gualberto, Jose M. ;

Prosper, Pascalita ;

Didierjean, Claude ;

Saul, Frederick ;

Haouz, Ahmed ;

Rouhier, Nicolas ;

Hecker, Arnaud .

BIOCHEMICAL JOURNAL, 2014, 462 :39-52

[15] Reduction of Benzoquinones to Hydroquinones via Spontaneous Reaction with Glutathione and Enzymatic Reaction by S-Glutathionyl-Hydroquinone Reductases [J].

Lam, L. K. Metthew ;

Zhang, Zhicheng ;

Board, Philip G. ;

Xun, Luying .

BIOCHEMISTRY, 2012, 51 (25) :5014-5021

[16] Functional Divergence of the Glutathione S-Transferase Supergene Family in Physcomitrella patens Reveals Complex Patterns of Large Gene Family Evolution in Land Plants [J].

Liu, Yan-Jing ;

Han, Xue-Min ;

Ren, Lin-Ling ;

Yang, Hai-Ling ;

Zeng, Qing-Yin .

PLANT PHYSIOLOGY, 2013, 161 (02) :773-786

[17] The Core of Chloroplast Nucleoids Contains Architectural SWIB Domain Proteins [J].

Melonek, Joanna ;

Matros, Andrea ;

Troesch, Mirl ;

Mock, Hans-Peter ;

Krupinska, Karin .

PLANT CELL, 2012, 24 (07) :3060-3073

[18] A single gene of chloroplast origin codes for mitochondrial and chloroplastic methionyl-tRNA synthetase in Arabidopsis thaliana [J].

Menand, B ;

Maréchal-Drouard, L ;

Sakamoto, W ;

Dietrich, A ;

Wintz, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (18) :11014-11019

[19] New substrates and activity of Phanerochaete chrysosporium Omega glutathione transferases [J].

Meux, Edgar ;

Morel, Melanie ;

Lamant, Tiphaine ;

Gerardin, Philippe ;

Jacquot, Jean-Pierre ;

Dumarcay, Stephane ;

Gelhaye, Eric .

BIOCHIMIE, 2013, 95 (02) :336-346

[20] Glutathione Transferases of Phanerochaete chrysosporium S-GLUTATHIONYL-p-HYDROQUINONE REDUCTASE BELONGS TO A NEW STRUCTURAL CLASS [J].

Meux, Edgar ;

Prosper, Pascalita ;

Ngadin, Andrew ;

Didierjean, Claude ;

Morel, Melanie ;

Dumarcay, Stephane ;

Lamant, Tiphaine ;

Jacquot, Jean-Pierre ;

Favier, Frederique ;

Gelhaye, Eric .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (11) :9162-9173

← 1 2 3 →