Measurement of the kinetic isotope effect for the oxidation of NADH at a poly(aniline)-modified electrode

被引：36

作者：

Bartlett, PN ^{[1
]}

Simon, E ^{[1
]}

机构：

[1] Univ Southampton, Dept Chem, Southampton SO17 1BJ, Hants, England

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 14期

关键词：

D O I：

10.1021/ja028943e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Kinetic isotope measurements using [4,4-2H2]NADH and [4-1H, 4-2H]NADH have been used to investigate the mechanism of the electrochemical oxidation of NADH at poly(aniline)-poly(vinyl sulfonate)-modified electrodes. The experiments show a primary kinetic isotope effect for the reaction of 4.2. This is consistent with literature values for the corresponding isotope effect for the oxidation of NADH by two-electron oxidants in homogeneous solution. The result demonstrates that transfer of H from NADH to the modified electrode occurs in the rate-limiting step within the reaction complex. Copyright © 2003 American Chemical Society.

引用

页码：4014 / 4015

页数：2

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