Endoplasmic Reticulum Stress Signals in Defined Human Embryonic Stem Cell Lines and Culture Conditions

被引：17
作者：
Angel Blanco-Gelaz, Miguel [1 ]
Suarez-Alvarez, Beatriz [1 ]
Ligero, Gertrudis [2 ]
Sanchez, Laura [2 ]
Ramon Vidal-Castineira, Jose [1 ]
Coto, Eliecer [3 ,5 ,6 ]
Moore, Harry [4 ]
Menendez, Pablo [2 ]
Lopez-Larrea, Carlos [1 ,5 ,6 ]
机构：
[1] Hosp Univ Cent Asturias, Histocompatibil & Transplantat Unit, Oviedo 33006, Spain
[2] Univ Granada, Inst Invest Biomed, Andalusian Stem Cell Bank, Granada, Spain
[3] Hosp Univ Cent Asturias, Dept Mol Genet, Oviedo 33006, Spain
[4] Univ Sheffield, Ctr Stem Cell Biol, Western Bank, Sheffield, S Yorkshire, England
[5] Inst Salud Carlos III, Madrid, Spain
[6] Fdn Renal Inigo Alvarez de Toledo, Madrid, Spain
来源：
STEM CELL REVIEWS AND REPORTS | 2010年 / 6卷 / 03期
关键词：
hESCs; Conditioned media; ER stress; Feeder-free culture system; UNFOLDED PROTEIN RESPONSE; GENE-EXPRESSION; DIFFERENTIATION; FIBROBLASTS; CHAPERONES; EXPANSION; FEEDERS; DEFENSE; GROWTH; ATF6;
D O I：
10.1007/s12015-010-9135-4
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Human embryonic stem cells (hESCs) are especially resistant to several cellular stresses, but the existence and induction of Endoplasmic Reticulum (ER) stress by culture conditions are unknown. Using qPCR, here, we investigated the behavior of the principal sensors of ER stress and their relation with the feeder layer, the type of conditioned media used in feeder free systems and the upregulation of several differentiation markers. We observed the preservation of pluripotency, and detected differential expression of differentiation markers in HS 181 and SHEF1 hESCs growing on Adipose-derived mesenchymal stem cells (ASCs) and feeder-free system with different conditioned media (HEF-CM and ASC-CM). Taken together, these results demonstrate evidence of ER stress events that cells must resolve to survive and maintenance of markers of pluripotency. The early differentiation status defined could progress into a more differentiated state, and may be influenced by culture conditions.
引用
收藏
页码：462 / 472
页数：11
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