Wnt10b Participates in Regulating Fatty Acid Synthesis in the Muscle of Zebrafish

There are 19 Wnt genes in mammals that belong to 12 subfamilies. Wnt signaling pathways participate in regulating numerous homeostatic and developmental processes in animals. However, the function of Wnt10b in fatty acid synthesis remains unclear in fish species. In the present study, we uncovered the role of the Wnt10b signaling pathway in the regulation of fatty acid synthesis in the muscle of zebrafish. The gene of Wnt10b was overexpressed in the muscle of zebrafish using pEGFP-N1-Wnt10b vector injection, which significantly decreased the expression of glycogen synthase kinase 3 beta (GSK-3 beta), but increased the expression of beta-catenin, peroxisome proliferators-activated receptor gamma (PPAR gamma), and CCAAT/enhancer binding protein alpha (C/EBP alpha). Moreover, the activity and mRNA expression of key lipogenic enzymes ATP-citrate lyase (ACL), acetyl-CoA carboxylase (ACC) and fatty acid synthetase (FAS), and the content of non-esterified fatty acids (NEFA), total cholesterol (TC), and triglyceride (TG) were also significantly decreased. Furthermore, interference of the Wnt10b gene significantly inhibited the expression of beta-catenin, PPAR gamma, and C/EBP alpha, but significantly induced the expression of GSK-3 beta, FAS, ACC, and ACL. The content of NEFA, TC, and TG as well as the activity of FAS, ACC, and ACL significantly increased. Thus, our results showed that Wnt10b participates in regulating fatty acid synthesis via beta-catenin, C/EBP alpha and PPAR gamma in the muscle of zebrafish.