Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry

被引：14

作者：

Dreher, MR ^{[1
]}

Elas, M

Ichikawa, K

Barth, ED

Chilkoti, A

Rosen, GM

Halpern, HJ

Dewhirst, M

机构：

[1] Duke Univ, Dept Biomed Engn, Durham, NC 27708 USA

[2] Univ Chicago, Ctr Low Frequency EPR Imaging In Vivo Physiol, Chicago, IL 60637 USA

[3] Univ Maryland, Sch Pharm, Dept Pharmaceut Sci, Baltimore, MD 21201 USA

[4] Univ Chicago, Dept Radiat & Cellular Oncol, Chicago, IL 60637 USA

[5] Jagiellonian Univ, Fac Biotechnol, Krakow, Poland

[6] Kyushu Univ, Fac Pharmaceut Sci, Dept Biophys, Fukuoka 812, Japan

来源：

MEDICAL PHYSICS | 2004年 / 31卷 / 10期

关键词：

thermometry; noninvasive system; biopolymer; elastin; electron paramagnetic resonance;

D O I：

10.1118/1.1782677

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Hyperthermia, as an adjuvant with radiation and chemotherapy, has shown promise in the treatment of cancer. The relevant biological effects of a hyperthermia treatment are both time and temperature-dependent, creating a need for accurate thermometry. We present a novel noninvasive thermometry modality that combines a temperature responsive biopolymer, the elastin-like polypeptide (ELP), and nitroxide to produce an ELP-nitroxide conjugate. When examined with electron paramagnetic resonance (EPR) spectroscopy, the ELP-nitroxide conjugate has temperature-dependent spectral line widths whose predictive accuracy is similar to0.3 degreesC (80 muM). We believe that the temperature-dependent changes observed in the EPR spectrum are due to the combined effect of temperature, viscosity and effective radius on the rotational correlation time of the ELP-nitroxide conjugate. (C) 2004 American Association of Physicists in Medicine.

引用

页码：2755 / 2762

页数：8