Alteration of intracellular free calcium and acylphosphatase levels in differentiating SH-SY5Y neuroblastoma cells

被引：0

作者：

Pieri, A ^{[1
]}

Liguri, G ^{[1
]}

Cecchi, C ^{[1
]}

Degl'Innocenti, D ^{[1
]}

Nassi, P ^{[1
]}

Ramponi, G ^{[1
]}

机构：

[1] Univ Florence, Dipartimento Sci Biochim, I-50134 Florence, Italy

来源：

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1997年 / 43卷 / 03期

关键词：

acylphosphatase; Differentiation; retinoic acid; free intracellular calcium; neuroblastoma; SH-SY5Y cells;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Levels of acylphosphatase isoenzymes and free intracellular calcium have been investigated in cultured SH-SY5Y human neuroblastoma cells under stimulation with all-trans retinoic acid and phorbol-12-myristate-13-acetate. Under these conditions morphological and functional characteristics demonstrated the differentiation of SH-SY5Y cells towards neuronal phenotype. Retinoic acid treatment caused a progressive and synchronous increase of the organ common-type acylphosphatase and of free intracellular calcium but not of the muscle-type acylphosphatase. Phorbol-12-myristate-13-acetate treatment gave rise to a peak of the muscle-type acylphosphatase levels during the early differentiation stage whereas organ common-type isoenzyme and free calcium levels show a pattern similar to that observed in retinoic acid-treated cells. These evidences indicate that the two acylphosphatase isoenzymes play different roles in SH-SY5Y differentiation and that during this process the expression of organ common-type acylphosphatase increases in a synchronous way with intracellular free calcium concentration.

引用

页码：633 / 641

页数：9

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