Interleukin-8 messenger ribonucleic acid expression correlates with tumor progression, tumor angiogenesis, patient survival, and timing of relapse in non-small-cell lung cancer

被引:140
作者
Yuan, A
Yang, PC
Yu, CJ
Chen, WJ
Lin, FY
Kuo, SH
Luh, KT
机构
[1] Natl Taiwan Univ Hosp, Dept Internal Med, Taipei 100, Taiwan
[2] Natl Taiwan Univ Hosp, Dept Surg, Taipei 100, Taiwan
[3] Natl Taiwan Univ Hosp, Dept Lab Med, Taipei 100, Taiwan
关键词
D O I
10.1164/ajrccm.162.5.2002108
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Tumor-associated angiogenesis is important for tumor growth and metastasis. Interleukin (IL)-8 was recently reported to be an important angiogenic factor both in vitro and in vivo. In this study we evaluated, for the first time, IL-8 messenger RNA (mRNA) expression in non-small-cell lung cancer (NSCLC), using real-time quantitative reverse-transcription-polymerase chain reaction, and correlated IL-8 mRNA expression in tumor and nontumor lung samples from 58 patients with NSCLC (29 with squamous cell carcinoma and 29 with adenocarcinoma, of whom 20 had Stage I, 10 had Stage II, and 28 had Stage III disease) with these patients' clinicopathologic characteristics, angiogenesis, and outcome. IL-8 protein expression and tumor microvessel count (MC) were assessed immunohistochemically. IL-8 mRNA expression was significantly greater in tumor tissue; high expression was highly associated with tumor in advanced stages (p = 0.03), distant lymph node metastasis (p = 0.02), high tumor MC (> 123) (p = 0.00003), short survival (< 26 mo) (p < 0.00001), and early relapse (< 16 mo) (p < 0.00001). Tumor MC correlated strongly with IL-8 mRNA expression (r = 0.56, p < 0.001). Multivariate analysis showed IL-8 mRNA expression and intratumor MC to be the most important predictors of patient survival and relapse. Thus, in NSCLC, IL-8 mRNA expression is strongly associated with tumor progression, tumor angiogenesis, survival, and time to relapse, suggesting its use as a prognostic indicator.
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页码:1957 / 1963
页数:7
相关论文
共 47 条
[11]   Regulation of interleukin-8 expression by reduced oxygen pressure in human glioblastoma [J].
Desbaillets, I ;
Diserens, AC ;
de Tribolet, N ;
Hamou, MF ;
Van Meir, EG .
ONCOGENE, 1999, 18 (07) :1447-1456
[12]   Upregulation of interleukin 8 by oxygen-deprived cells in glioblastoma suggests a role in leukocyte activation, chemotaxis, and angiogenesis [J].
Desbaillets, I ;
Diserens, AC ;
deTribolet, N ;
Hamou, MF ;
Van Meir, EG .
JOURNAL OF EXPERIMENTAL MEDICINE, 1997, 186 (08) :1201-1212
[13]   Role of angiogenic factors: Coexpression of interleukin-8 and vascular endothelial growth factor in patients with head and neck squamous carcinoma [J].
Eisma, RJ ;
Spiro, JD ;
Kreutzer, DL .
LARYNGOSCOPE, 1999, 109 (05) :687-693
[14]   Angiogenesis and prostate cancer: In vivo and in vitro expression of angiogenesis factors by prostate cancer cells [J].
Ferrer, FA ;
Miller, LJ ;
Andrawis, RI ;
Kurtzman, SH ;
Albertsen, PC ;
Laudone, VP ;
Kreutzer, DL .
UROLOGY, 1998, 51 (01) :161-167
[15]   Real-time quantitative RT-PCR after laser-assisted cell picking [J].
Fink, L ;
Seeger, W ;
Ermert, L ;
Hänze, J ;
Stahl, U ;
Grimminger, F ;
Kummer, W ;
Bohle, RM .
NATURE MEDICINE, 1998, 4 (11) :1329-1333
[16]  
FOLKMAN J, 1992, J BIOL CHEM, V267, P10931
[17]   TUMOR ANGIOGENESIS [J].
FOLKMAN, J .
ADVANCES IN CANCER RESEARCH, 1985, 43 :175-203
[18]   Seminars in medicine of the Beth Israel Hospital, Boston - Clinical applications of research on angiogenesis [J].
Folkman, J .
NEW ENGLAND JOURNAL OF MEDICINE, 1995, 333 (26) :1757-1763
[19]   MOLECULAR MECHANISMS OF ANGIOGENESIS - FIBROBLAST GROWTH-FACTOR SIGNAL-TRANSDUCTION [J].
FRIESEL, RE ;
MACIAG, T .
FASEB JOURNAL, 1995, 9 (10) :919-925
[20]   Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis [J].
Hanahan, D ;
Folkman, J .
CELL, 1996, 86 (03) :353-364