pH-responsive materials for optical monitoring of wound status

被引:40
作者
Gamerith, Clemens [1 ,2 ,3 ]
Luschnig, Daniel [1 ,2 ]
Ortner, Andreas [3 ]
Pietrzik, Nikolas [4 ]
Guse, Jan-Hinrich [4 ]
Burnet, Michael [2 ,4 ]
Haalboom, Marieke [5 ,6 ]
van der Palen, Job [5 ]
Heinzle, Andrea [2 ]
Sigl, Eva [2 ]
Gubitz, Georg M. [1 ,3 ]
机构
[1] ACIB, Petersgasse 14, A-8010 Graz, Austria
[2] Qualizyme Diagnost GmbH & Co KG, Neue Stiftingtalstr 2, A-8010 Graz, Austria
[3] Univ Nat Resources & Life Sci Vienna, Dept Environm Biotechnol, Konrad Lorenz Str 20, A-3430 Tulln An Der Donau, Austria
[4] Synovo GmbH, Paul Ehrlich Str 15, D-72076 Tubingen, Germany
[5] Med Spectrum Twente Hosp, Med Sch Twente, POB 50 000, NL-7500 KA Enschede, Netherlands
[6] Med Spectrum Twente Hosp, Dept Surg, POB 50 000, NL-7500 KA Enschede, Netherlands
关键词
Immobilized pH indicator; Visual indicator; Diagnostic materials; Wound pH; Infection risk; WIRELESS CONNECTIVITY; SENSOR MATERIALS; SMART BANDAGE; LOW-COST; INDICATOR; INFECTION; FIBERS; DYES;
D O I
10.1016/j.snb.2019.126966
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The monitoring of infection status of wounds is an emerging field and the pH of wound exudate is considered one potential indicator of infection. pH indicators intended for use in medical devices, such as swabs or dressings, need to be fixed in place, however, visual pH indicators are usually soluble molecules so are not inherently suitable for use in devices. To address this, we developed a rapid and simple immobilisation method for coupling pH-responsive dyes onto solid phases. The use of a silane based coupling agent for immobilisation of bromocresol purple led to a shift in the pH dependent spectral properties of the resulting material. The pH responsive material changes from yellow to green to blue with rising pH providing an ideal contrast to the reddish colour of most wound exudates. This is a key advantage over currently available alternatives when considering the suitability of this material for incorporation into various medical devices. In addition, we analysed clinical study samples to verify the association between wound infection and elevated pH-values. A device with an embedded indicator that changes to a contrast colour could represent a simple and easy-to-use system for detecting wounds at risk of infection.
引用
收藏
页数:9
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