Windrow composting mitigated CH4 emissions: characterization of methanogenic and methanotrophic communities in manure management

被引:41
作者
Chen, Ruirui [1 ]
Wang, Yiming [1 ]
Wei, Shiping [1 ]
Wang, Wei [1 ]
Lin, Xiangui [1 ]
机构
[1] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
compost; manure; methanogens; methanotrophs; pyrosequencing; qPCR; GREENHOUSE-GAS EMISSIONS; MICROBIAL METHANE PRODUCTION; CATTLE FEEDLOT MANURE; NITROUS-OXIDE; RICE FIELD; ARCHAEAL COMMUNITIES; PCR-DGGE; DAIRY; TEMPERATURE; NH3;
D O I
10.1111/1574-6941.12417
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
With increasing livestock breeding, methane (CH4) emissions from manure management will increasingly contribute more to atmospheric CH4 concentration. The dynamics of methanogens and methanotrophs have not yet been studied in the manure environment. The current study combines surface CH4 emissions with methanogenic and methanotrophic community analyses from two management practices, windrow composting (WCOM) and solid storage (SSTO). Our results showed that there was an c. 50% reduction of CH4 emissions with WCOM compared with SSTO over a 50-day period. A sharp decrease in the quantities of both methanogens and methanotrophs in WCOM suggested that CH4 mitigation was mainly due to decreased CH4 production rather than increased CH4 oxidation. Pyrosequencing analysis demonstrated that aeration caused a clear shift of dominant methanogens in the manure, with specifically a significant decrease in Methanosarcina and increase in Methanobrevibacter. The composition of methanogenic community was influenced by manure management and regulated CH4 production. A sharp increase in the quantity of methanotrophs in SSTO suggested that microbial CH4 oxidation is an important sink for the CH4 produced. The increased abundance of Methylococcaceae in SSTO suggested that Type I methanotrophs have an advantage in CH4 oxidation in occupying niches under low CH4 and high O-2 conditions.
引用
收藏
页码:575 / 586
页数:12
相关论文
共 61 条
  • [1] Pile mixing increases greenhouse gas emissions during composting of dairy manure
    Ahn, H. K.
    Mulbry, W.
    White, J. W.
    Kondrad, S. L.
    [J]. BIORESOURCE TECHNOLOGY, 2011, 102 (03) : 2904 - 2909
  • [2] GROWTH OF METHANOTROPHS IN METHANE AND OXYGEN COUNTER GRADIENTS
    AMARAL, JA
    KNOWLES, R
    [J]. FEMS MICROBIOLOGY LETTERS, 1995, 126 (03) : 215 - 220
  • [3] Emissions of NH3, N2O and CH4 from dairy cows housed in a farmyard manure tying stall (housing, manure storage, manure spreading)
    Amon, B
    Amon, T
    Boxberger, J
    Alt, C
    [J]. NUTRIENT CYCLING IN AGROECOSYSTEMS, 2001, 60 (1-3) : 103 - 113
  • [4] Quantification of Greenhouse Gas Emissions from Windrow Composting of Garden Waste
    Andersen, Jacob K.
    Boldrin, Alessio
    Samuelsson, Jerker
    Christensen, Thomas H.
    Scheutz, Charlotte
    [J]. JOURNAL OF ENVIRONMENTAL QUALITY, 2010, 39 (02) : 713 - 724
  • [5] Methanogenic archaea are globally ubiquitous in aerated soils and become active under wet anoxic conditions
    Angel, Roey
    Claus, Peter
    Conrad, Ralf
    [J]. ISME JOURNAL, 2012, 6 (04) : 847 - 862
  • [6] Activation of Methanogenesis in Arid Biological Soil Crusts Despite the Presence of Oxygen
    Angel, Roey
    Matthies, Diethart
    Conrad, Ralf
    [J]. PLOS ONE, 2011, 6 (05):
  • [7] METHANOGENS - RE-EVALUATION OF A UNIQUE BIOLOGICAL GROUP
    BALCH, WE
    FOX, GE
    MAGRUM, LJ
    WOESE, CR
    WOLFE, RS
    [J]. MICROBIOLOGICAL REVIEWS, 1979, 43 (02) : 260 - 296
  • [8] A metagenomic insight into freshwater methane-utilizing communities and evidence for cooperation between the Methylococcaceae and the Methylophilaceae
    Beck, David A. C.
    Kalyuzhnaya, Marina G.
    Malfatti, Stephanie
    Tringe, Susannah G.
    del Rio, Tijana Glavina
    Ivanova, Natalia
    Lidstrom, Mary E.
    Chistoserdova, Ludmila
    [J]. PEERJ, 2013, 1
  • [9] Composting of animal manures and chemical criteria for compost maturity assessment. A review
    Bernal, M. P.
    Alburquerque, J. A.
    Moral, R.
    [J]. BIORESOURCE TECHNOLOGY, 2009, 100 (22) : 5444 - 5453
  • [10] The Positive Effect of Exogenous Hemin on a Resistance of Strict Anaerobic Archaeon Methanobrevibacter arboriphilus to Oxidative Stresses
    Brioukhanov, Andrei L.
    Netrusov, Alexander I.
    [J]. CURRENT MICROBIOLOGY, 2012, 65 (04) : 375 - 383