Glycomyces dulcitolivorans sp nov., isolated from rhizosphere soil of wheat (Triticum aestivum L.)

A novel actinomycete, designated strain SJ-25(T), was isolated from rhizosphere soil of wheat (Triticumaestivum L.) and characterized using a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain SJ-25(T) belonged to the genus Glycomyces and was closely related to Glycomyces scopariae YIM 56256(T) (99.0% 16S rRNA gene sequence similarity), Glycomyces artemisiae IXS4(T) (98.8 %), Glycomyces sambucus E71(T) (98.7 %) and Glycomyces mayteni YIM 61331(T) (98.4 %). Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain SJ-25(T) to the genus Glycomyces. The cell wall contained meso-diaminopimelic acid and the whole-cell hydrolysates contained galactose, glucose and xylose. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, glycolipid and two unidentified polar lipids. The menaquinones were MK-11, MK-10(H-4) and MK-10(H-2). Major fatty acids were iso-C-16 : 0, anteiso-C-15 : 0, iso-C-15 : 0, anteiso-C-17 : 0 and iso-C-14 : 0. The DNA G+C content was 72.2 mol%. The combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain SJ-25(T) could be distinguished from its closely related strains. Therefore, it is proposed that strain SJ-25(T) represents a novel species of the genus Glycomyces, for which the name Glycomyces dulcitolivorans sp. nov. is proposed. The type strain is SJ-25(T) (= CGMCC 4.7414(T) = DSM 105121(T)).