The Recombinant Maize Ribosome-Inactivating Protein Transiently Reduces Viral Load in SHIV89.6 Infected Chinese Rhesus Macaques

被引：9

作者：

Wang, Rui-Rui ^{[1
]}

Au, Ka-Yee ^{[2
]}

Zheng, Hong-Yi ^{[1
]}

Gao, Liang-Min ^{[3
]}

Zhang, Xuan ^{[1
]}

Luo, Rong-Hua ^{[1
]}

Law, Sue Ka-Yee ^{[2
]}

Mak, Amanda Nga-Sze ^{[2
]}

Wong, Kam-Bo ^{[2
]}

Zhang, Ming-Xu ^{[1
]}

Pang, Wei ^{[1
]}

Zhang, Gao-Hong ^{[1
]}

Shaw, Pang-Chui ^{[2
]}

Zheng, Yong-Tang ^{[1
]}

机构：

[1] Chinese Acad Sci, Key Lab Anim Models & Human Dis Mech, Chinese Acad Sci & Yunnan Prov, Kunming Inst Zool, Kunming 650223, Yunnan, Peoples R China

[2] Chinese Univ Hong Kong, Ctr Prot Sci & Crystallog, Sch Life Sci, Shatin 999077, Hong Kong, Peoples R China

[3] Yuxi Ctr Dis Control & Prevent, Div HIV AIDS & STI Control, Yuxi 653100, Yunnan, Peoples R China

来源：

TOXINS | 2015年 / 7卷 / 01期

关键词：

maize RIP; anti-HIV; animal model; viral load; HUMAN-IMMUNODEFICIENCY-VIRUS; ANTI-HIV; ANTIVIRAL PROTEIN; AIDS; RNA; RESISTANCE; INHIBITOR; MONKEYS; COUNTS; MAP30;

D O I：

10.3390/toxins7010156

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Ribosome inactivating proteins (RIPs) inhibit protein synthesis by depurinating the large ribosomal RNA and some are found to possess anti-human immunodeficiency virus (HIV) activity. Maize ribosome inactivating protein (RIP) has an internal inactivation loop which is proteolytically removed for full catalytic activity. Here, we showed that the recombinant active maize RIP protected chimeric simian-human immunodeficiency virus (SHIV) 89.6-infected macaque peripheral blood mononuclear cells from lysis ex vivo and transiently reduced plasma viral load in SHIV89.6-infected rhesus macaque model. No evidence of immune dysregulation and other obvious side-effects was found in the treated macaques. Our work demonstrates the potential development of maize RIP as an anti-HIV agent without impeding systemic immune functions.

引用

页码：156 / 169

页数：14

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