Neospora caninum: Cloning and expression of a gene coding for cytokine-inducing profilin

被引：20

作者：

Jenkins, Mark C. ^{[1
]}

Tuo, Wenbin ^{[1
]}

Feng, Xiaosheng ^{[1
]}

Cao, Lili ^{[1
]}

Murphy, Charles ^{[2
]}

Fetterer, Raymond ^{[1
]}

机构：

[1] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA

[2] ARS, Soybean Genet Improvement Lab, USDA, Beltsville, MD 20705 USA

来源：

EXPERIMENTAL PARASITOLOGY | 2010年 / 125卷 / 04期

关键词：

Neospora caninum; Profilin; Tachyzoites; Cytokine response; HOST-CELL INVASION; TOXOPLASMA-GONDII; VERTICAL TRANSMISSION; PLASMODIUM-FALCIPARUM; RECEPTOR RECOGNITION; RECOMBINANT NCSRS2; SURFACE PROTEIN; MICE; IMMUNIZATION; INFECTION;

D O I：

10.1016/j.exppara.2010.03.001

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Profilins are actin-binding proteins that in Toxoplasma gondii stimulate innate immunity in mice by binding Toll-like receptors (TLR) on dendritic cells (DC) leading to release of inflammatory cytokines, primarily IL-12 and IFN-gamma. The purpose of the present study was to characterize Neospora caninum profilin, termed NcProfilin. Recombinant NcProfilin was purified by affinity chromatography, and used to prepare specific antisera to allow characterization of native NcProfilin antigen in N. caninum tachyzoites. By immunoblotting, recombinant NcProfilin is 22 kDa, and is similar in size to the respective 22 kDa native protein. Immunofluorescence and immunoelectron microscopy localized native NcProfilin to the apical end of N. caninum tachyzoites. Incubation of recombinant NcProfilin with spleen cells from BALB/c mice induced release of IFN-gamma. Also, injection of BALB/c mice with purified rNcProfilin elicited a strong IFN-gamma and IL-12 responses at 6 and 24 h after injection indicating that NcProfilin may be an important protein in regulation of cytokine responses to N. caninum. Published by Elsevier Inc.

引用

页码：357 / 362

页数：6

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