A new tool for superoxide and nitric oxide radicals determination using suitable enzymatic sensors

被引：37
作者：
Campanella, L [1 ]
Persi, L [1 ]
Tomassetti, M [1 ]
机构：
[1] Univ Rome La Sapienza, Dept Chem, I-00185 Rome, Italy
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2000年 / 68卷 / 1-3期
关键词：
superoxide; nitric oxide; radicals; detection; biosensors;
D O I：
10.1016/S0925-4005(00)00415-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new tool for radical determination is investigated. Superoxide and nitric oxide radicals have been determined using their modulating action on three different enzymatic sensors based on tyrosinase, superoxide dismutase (SOD), and galactose oxidase (GAO), respectively. Basic research was carried nut to study the inhibition or activation effect of the two radicals considered on the enzyme sensor's response and experimentally evidencing the correlation between the modulating action of the biosensor response and the radical concentration in solution. Results are shown as linear ranges, which differ according to the biosensor used and the radical determined. (C) 2000 Elsevier Science S.A. All rights reserved.
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收藏
页码：351 / 359
页数：9
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