Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo

被引:48
作者
Lin, Ziwei [1 ]
Gan, Tiantian [1 ]
Huang, Yanzhen [1 ]
Bao, Lijun [1 ]
Liu, Shuang [1 ]
Cui, Xiaopeng [1 ]
Wang, Hexin [1 ]
Jiao, Feng [1 ]
Zhang, Minjuan [1 ]
Su, Chao [1 ]
Qian, Yonghua [1 ]
机构
[1] Northwest A&F Univ, Coll Anim Sci & Technol, Sericultural & Silk Res Inst, Xianyang 712100, Peoples R China
关键词
mulberry leaf; flavonoids; anti-inflammatory; LC-MS; ulcerative colitis; MORUS-ALBA L; INFLAMMATORY-BOWEL-DISEASE; NITRIC-OXIDE; OXIDATIVE STRESS; ETHANOL EXTRACT; INDUCED COLITIS; MOUSE MODEL; ANTIOXIDANT; LEAVES; HEALTH;
D O I
10.3390/ijms23147694
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mulberry (Morus alba L.) is a flowering tree traditionally used in Chinese herbal medicine. Mulberry leaf flavonoids (MLFs) have been reported to exert important anti-inflammatory and antioxidant properties. The purpose of this study was to select the MLF with the best anti-inflammatory and antioxidative activities from MLFs eluted by different ethanol concentrations (30%, 50%, and 75%) and explore its pharmacological properties. Three types of MLFs inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines in lipopolysaccharide (LPS)-induced RAW 264.7 cells. All MLFs boosted the antioxidative capacity by decreasing the reactive oxygen species (ROS) production and the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and improving the metal ion chelating activity and reducing power. The results revealed that the MLFs eluted by 30% ethanol exhibited the best anti-inflammatory and antioxidative activities. A nontargeted metabolomic analysis was used to analyze 24 types of differential flavonoids between the MLFs. Quercetin, kaempferol, and their derivatives in 30%MLF were more abundant than the other two MLFs. Furthermore, we evaluated the pharmacological activities of 30%MLF in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice. The 30%MLF could alleviate the clinical symptoms, reduce the secretion of inflammatory cytokines, and inhibit the activation of the inflammatory pathway in DSS-induced colitis mice. This study will provide valuable information for the development of MLFs eluted by 30% ethanol as a functional food.
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页数:22
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