Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit

被引:93
作者
Ninet, B
Jan, I
Bontems, O
Léchenne, B
Jousson, O
Panizzon, R
Lew, D
Monod, M
机构
[1] CHU Vaudois, Serv Dermatol, Lab Mycol, DHURDV, CH-1011 Lausanne, Switzerland
[2] Hop Cantonal Geneva, Div Malad Infect, CH-1211 Geneva, Switzerland
关键词
D O I
10.1128/JCM.41.2.826-830.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.
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收藏
页码:826 / 830
页数:5
相关论文
共 16 条
[1]   Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts [J].
Chen, YC ;
Eisner, JD ;
Kattar, MM ;
Rassoulian-Barrett, SL ;
Lafe, K ;
Bui, U ;
Limaye, AP ;
Cookson, BT .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (11) :4042-4051
[2]   Application of PCR to distinguish common species of dermatophytes [J].
Faggi, E ;
Pini, G ;
Campisi, E ;
Bertellini, C ;
Difonzo, E ;
Mancianti, F .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (09) :3382-3385
[3]   Phylogeny and taxonomy of the family Arthrodermataceae (dermatophytes) using sequence analysis of the ribosomal ITS region [J].
Gräser, Y ;
El Fari, M ;
Vilgalys, R ;
Kuijpers, AFA ;
De Hoog, GS ;
Presber, W ;
Tietz, HJ .
MEDICAL MYCOLOGY, 1999, 37 (02) :105-114
[4]   Molecular and conventional taxonomy of the Microsporum canis complex [J].
Gräser, Y ;
Kuijpers, AFA ;
El Fari, M ;
Presber, W ;
De Hoog, GS .
MEDICAL MYCOLOGY, 2000, 38 (02) :143-153
[5]   Molecular taxonomy of Trichophyton mentagrophytes and T-tonsurans [J].
Gräser, Y ;
Kuijpers, AFA ;
Presber, W ;
De Hoog, GS .
MEDICAL MYCOLOGY, 1999, 37 (05) :315-330
[6]  
Hall T.A., 1999, BIOEDIT USER, V41, P95, DOI 10.1021/bk-1999-0734.ch008
[7]  
HARMSEN D, 1995, J MED VET MYCOL, V33, P299
[8]   Identification of Aspergillus species using internal transcribed spacer regions 1 and 2 [J].
Henry, T ;
Iwen, PC ;
Hinrichs, SH .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (04) :1510-1515
[9]  
Horguelin P.A., 1978, PRATIQUE REVISION
[10]  
Kwong-Chung K. J., 1992, MED MYCOLOGY