Sensitive fluorescent imaging of Salmonella enteritidis and Salmonella typhimurium using a polyvalent directed peptide polymer

被引：21

作者：

Lee, Sang-Choon ^{[1
,2
]}

Kim, Min-Seo ^{[1
,2
]}

Yoo, Ki-Chun ^{[3
]}

Ha, Na-Reum ^{[1
,2
]}

Moon, Ji-Young ^{[1
,2
]}

Lee, Su-Jae ^{[3
]}

Yoon, Moon-Young ^{[1
,2
]}

机构：

[1] Hanyang Univ, Dept Chem, 222 Wangsimni Ro, Seoul 04763, South Korea

[2] Hanyang Univ, Res Inst Nat Sci, 222 Wangsimni Ro, Seoul 04763, South Korea

[3] Hanyang Univ, Res Inst Nat Sci, Dept Life Sci, 222 Wangsimni Ro, Seoul 04763, South Korea

来源：

MICROCHIMICA ACTA | 2017年 / 184卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

Food poisoning; Cell-phage display; Polyvalant directed peptide polymer; Fluorescein isothiocyanate; Succinimidyl 3-(2-pyridyldithio)propionate; RT-PCR; ANTHRAX-PROTECTIVE ANTIGEN; RAPID DETECTION; IDENTIFICATION; BINDING; AMPLIFICATION; SELECTION; APTAMERS; BACTERIA; LIGANDS; PROBES;

D O I：

10.1007/s00604-017-2240-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The authors describe three fluorescein-conjugated peptides generated by cell-phage display for use as a diagnostic probes for fluorescent detection and imaging of Salmonella enteritidis and Salmonella typhimurium. The authors also designed a polyvalent-directed peptide polymer synthesized with poly-D-lysine and bifunctional succinimidyl 3-(2pyridyldithio) propionate with an affinity and sensitivity that is higher by more than an order of magnitude compared to single peptides due to multiple binding site interactions. In order to establish a diagnostic system for food poisoning, imaging analysis was performed using fluorescence microscopy. The limit of detection of the diagnostic system based on polyvalent directed peptide interaction is 10(2) colony-forming units per mL for Salmonella.

引用

页码：2611 / 2620

页数：10

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