Synthesis of photoactivatable azido-acyl caged oxazine fluorophores for live-cell imaging

被引：17

作者：

Anzalone, Andrew V. ^{[1
,2
]}

Chen, Zhixing ^{[1
,3
]}

Cornish, Virginia W. ^{[1
,2
]}

机构：

[1] Columbia Univ, Dept Chem, New York, NY 10027 USA

[2] Columbia Univ, Dept Syst Biol, New York, NY 10032 USA

[3] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 60期

基金：

美国国家卫生研究院;

关键词：

FLUORESCENT-PROBES; GENERAL-METHOD; CHEMICAL TAGS; LIVING CELLS; IN-VIVO; DYES; MICROSCOPY; DERIVATIVES; PROTEINS;

D O I：

10.1039/c6cc04882j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the design and synthesis of a photoactivatable azidoacyl oxazine fluorophore. Photoactivation is achieved cleanly and rapidly with UV light, producing a single fluorescent oxazine photoproduct. We demonstrate the utility of azido-acyl caged oxazines for protein specific labeling in living mammalian cells using the TMP-tag technology.

引用

页码：9442 / 9445

页数：4

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