Genetic incorporation of 1,2-aminothiol functionality for site-specific protein modification via thiazolidine formation

被引：21

作者：

Bi, Xiaobao ^{[1
]}

Pasunooti, Kalyan Kumar ^{[1
]}

Tareq, Ahmad Hussen ^{[1
]}

Takyi-Williams, John ^{[1
]}

Liu, Chuan-Fa ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Biol Sci, Div Struct Biol & Biochem, 60 Nanyang Dr, Singapore 637551, Singapore

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2016年 / 14卷 / 23期

关键词：

AMINO-ACID; UBIQUITIN; LIGATION; CHEMISTRY; CONJUGATION; PEPTIDES; ALDEHYDE; STRATEGY; ANALOGS; BOND;

D O I：

10.1039/c6ob00854b

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Here we report a new site-specific conjugation strategy to modify proteins via thiazolidine ligation. Proteins harbouring a 1,2-aminothiol moiety introduced by amber codon suppression technology could be modified chemoselectively with aldehyde-functionalized reagents, such as a biotin-labeled peptide or ubiquitin, under mild conditions to yield homogeneous biotinylated or ubiquitinated products.

引用

页码：5282 / 5285

页数：4

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