Effect of Desiccating Stress on Mouse Meibomian Gland Function

被引:59
作者
Suhalim, Jeffrey L. [1 ,2 ,3 ]
Parfitt, Geraint J. [1 ]
Xie, Yilu [1 ]
De Paiva, Cintia S. [4 ]
Pflugfelder, Stephen C. [4 ]
Shah, Tejas N. [1 ]
Potma, Eric O. [2 ]
Brown, Donald J. [1 ]
Jester, James V. [1 ,3 ]
机构
[1] Univ Calif Irvine, Gavin Herbert Eye Inst, Irvine, CA 92697 USA
[2] Univ Calif Irvine, Beckman Laser Inst & Med Clin, Irvine, CA 92697 USA
[3] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA 92697 USA
[4] Baylor Coll Med, Ocular Surface Ctr, Dept Ophthalmol, Houston, TX 77030 USA
基金
美国国家卫生研究院;
关键词
evaporative dry eye; meibomian gland; nonlinear optical microscopy; stimulated Raman scattering; STIMULATED RAMAN-SCATTERING; PRINCIPAL COMPONENT ANALYSIS; MEIBUM LIPID-COMPOSITION; INTERNATIONAL WORKSHOP; DRY EYE; DYSFUNCTION REPORT; IN-VIVO; OCULAR SURFACE; MICROSCOPY; SUBCOMMITTEE;
D O I
10.1016/j.jtos.2013.08.002
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose: Mice exposed to standardized desiccating environmental stress to induce dry eye-like symptoms have been used as a model to study the underlying mechanisms of evaporative dry eye. While studies have shown marked inflammatory and immune changes, the effect of such stress on meibomian gland function remains largely unknown. We sought to evaluate the effects of desiccating stress on meibocyte proliferation and meibum quality. Methods: Ten mice were treated with scopolamine and subjected to a drafty low humidity environment (30-35%). Five and ten days after treatment, eyelids were harvested and cryosections stained with Ki67 antibody to identify cycling cells. Sections were also imaged using stimulated Raman scattering (SRS) microscopy to characterize the gland compositional changes by detecting the vibrational signatures of methylene (lipid) and amide-I (protein). Results: Desiccating stress caused a 3-fold increase in basal acinar cell proliferation from 18.3 +/- 11.1% in untreated mice to 64.4 +/- 19.9% and 66.6 +/- 13.4% after 5 and 10 days exposure, respectively (P < .001). In addition, SRS analysis showed a wider variation in the protein-to-lipid ratio throughout the gland, suggesting alterations in meibocyte differentiation and lipid synthesis. Conclusions: These data are consistent with a model that a desiccating environment may have a direct effect on meibomian gland function, leading to a significant increase in basal acinar cell proliferation, abnormal meibocyte differentiation, and altered lipid production.
引用
收藏
页码:59 / 68
页数:10
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