Molecular markers of resistance to sulphadoxine-pyrimethamine one year after implementation of intermittent preventive treatment of malaria in infants in Mali

被引:36
作者
Dicko, Alassane [1 ,2 ]
Sagara, Issaka [1 ]
Djimde, Abdoulaye A. [1 ]
Toure, Sidy O. [1 ]
Traore, Mariam [1 ]
Dama, Souleymane [1 ]
Diallo, Abdoulbaki I. [1 ]
Barry, Amadou [1 ]
Dicko, Mohamed [1 ]
Coulibaly, Oumar M. [3 ]
Rogier, Christophe [4 ]
de Sousa, Alexandra [5 ]
Doumbo, Ogobara K. [1 ]
机构
[1] Univ Bamako, Fac Med Pharm & Dent, Dept Epidemiol Parasit Dis, Malaria Res & Training Ctr, Bamako, Mali
[2] Univ Bamako, Fac Med Pharm & Dent, Dept Publ Hlth, Bamako, Mali
[3] Ctr Sante Reference Kolokani, Region De Koulikoro, Mali
[4] URMITE, UMR6236, Ex IMTSSA, IRBA, F-13262 Marseille 07, France
[5] UNICEF Head Quarters, New York, NY 10017 USA
关键词
FALCIPARUM DIHYDROFOLATE-REDUCTASE; PLASMODIUM-FALCIPARUM; RAPID INCREASE; DOUBLE-BLIND; ROUTINE VACCINATIONS; TREATMENT FAILURE; DRUG-RESISTANCE; MUTATIONS; SELECTION; AMODIAQUINE;
D O I
10.1186/1475-2875-9-9
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background: Intermittent preventive treatment in infants (IPTi) with sulphadoxine-pyrimethamine (SP) given during routine vaccinations is efficacious in preventing malaria disease and shows no interaction with the vaccines. However, there is a fear that IPTi may result in a rapid increase of parasite resistance to SP. Methods: To evaluate the impact of IPTi on SP-resistance point mutations, the 22 health sub-districts in the district of Kolokani, Mali, were randomized in a 1: 1 ratio and starting in December 2006, IPTi with SP was implemented in 11 health sub-districts (intervention zone), while the other 11 health sub-districts served as the control (nonintervention zone). Blood smears and blood dots on filter paper were obtained from children aged 0-5 years, randomly selected in each of heath sub-districts during two cross-sectional surveys. The first survey was conducted in May 2007 before the start of the transmission season to collect baseline prevalence of the molecular markers of resistance to SP and the second in December 2007 after the end of the transmission season and one year after implementation of IPTi. A total of 427 and 923 randomly selected blood samples from the first and second surveys respectively were analysed by PCR for dhfr and dhps mutations. Results: Each of the three dhfr mutations at codons 51, 59 and 108 was present in 35% and 57% of the samples during the two surveys with no significant differences between the two zones. Dhps mutations at codons 437 and 540 were present respectively in about 20% and 1% of the children during the two surveys in both zones at similar proportion. The prevalence of quadruple mutants (triple dhfr-mutants + dhps-437G) associated with in-vivo resistance to SP in Mali after one year implementation of IPTi was also similar between the two zones (11.6% versus 11.2%, p = 0.90) and to those obtained at baseline survey (10.3% versus 8.1%). Conclusion: This study shows no increase in the frequency of molecular markers of SP resistance in areas where IPTi with SP was implemented for one year.
引用
收藏
页数:7
相关论文
共 35 条
[1]  
Akhwale W, 2008, LANCET, V372, P1383, DOI 10.1016/S0140-6736(08)61583-4
[2]   Modelling the impact of intermittent preventive treatment for malaria on selection pressure for drug resistance [J].
Alexander, Neal ;
Sutherland, Colin ;
Roper, Cally ;
Cisse, Badara ;
Schellenberg, David .
MALARIA JOURNAL, 2007, 6 (1)
[3]   Efficacy and safety of intermittent preventive treatment with sulfadoxine-pyrimethamine for malaria in African infants: a pooled analysis of six randomised, placebo-controlled trials [J].
Aponte, John J. ;
Schellenberg, David ;
Egan, Andrea ;
Breckenridge, Alasdair ;
Carneiro, Ilona ;
Critchley, Julia ;
Danquah, Ina ;
Dodoo, Alexander ;
Kobbe, Robin ;
Lell, Bertrand ;
May, Juergen ;
Premji, Zul ;
Sanz, Sergi ;
Sevene, Esperanza ;
Soulaymani-Becheikh, Rachida ;
Winstanley, Peter ;
Adjei, Samuel ;
Anemana, Sylvester ;
Chandramohan, Daniel ;
Issifou, Saadou ;
Mockenhaupt, Frank ;
Owusu-Agyei, Seth ;
Greenwood, Brian ;
Grobusch, Martin P. ;
Kremsner, Peter G. ;
Macete, Eusebio ;
Mshinda, Hassan ;
Newman, Robert D. ;
Slutsker, Laurence ;
Tanner, Marcel ;
Alonso, Pedro ;
Menendez, Clara .
LANCET, 2009, 374 (9700) :1533-1542
[4]  
Bogreau H, 2006, AM J TROP MED HYG, V74, P953
[5]   Cluster randomised trial of intermittent preventive treatment for malaria in infants in area of high, seasonal transmission in Ghana [J].
Chandramohan, D ;
Owusu-Agyei, S ;
Carneiro, I ;
Awine, T ;
Amponsa-Achiano, K ;
Mensah, N ;
Jaffar, S ;
Baiden, R ;
Hodgson, A ;
Binka, F ;
Greenwood, B .
BMJ-BRITISH MEDICAL JOURNAL, 2005, 331 (7519) :727-733
[6]   Seasonal intermittent preventive treatment with artesunate and sulfadoxine-pyrimethamine for prevention of malaria in Senegalese children:: a randomised, placebo-controlled, double-blind trial [J].
Cissé, B ;
Sokhna, C ;
Boulanger, D ;
Milet, J ;
Bâ, EH ;
Richardson, K ;
Hallett, R ;
Sutherland, C ;
Simondon, K ;
Simondon, F ;
Alexander, N ;
Gaye, O ;
Targett, G ;
Lines, J ;
Greenwood, B ;
Trape, JF .
LANCET, 2006, 367 (9511) :659-667
[7]   Sustained use of insecticide-treated curtains is not associated with greater circulation of drug-resistant malaria parasites, or with higher risk of treatment failure among children with uncomplicated malaria in Burkina Faso [J].
Diallo, Diadier A. ;
Sutherland, Colin ;
Nebie, Issa ;
Konate, Amadou T. ;
Ord, Rosalynn ;
Pota, Hirva ;
Roper, Cally ;
Ilboudo-Sanogo, Edith ;
Greenwood, Brian M. ;
Cousens, Simon N. .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2007, 76 (02) :237-244
[8]   Impact of intermittent preventive treatment with sulphadoxine-pyrimethamine targeting the transmission season on the incidence of clinical malaria in children in Mali [J].
Dicko, Alassane ;
Sagara, Issaka ;
Sissoko, Mahamadou S. ;
Guindo, Ousmane ;
Diallo, Abdoulbaki I. ;
Kone, Mamady ;
Toure, Ousmane B. ;
Sacko, Massambou ;
Doumbo, Ogobara K. .
MALARIA JOURNAL, 2008, 7 (1)
[9]  
Djimdé A, 2001, NEW ENGL J MED, V344, P257, DOI 10.1056/NEJM200101253440403
[10]   Efficacy, safety, and selection of molecular markers of drug resistance by two ACTs in Mali [J].
Djimde, Abdoulaye A. ;
Fofana, Bakary ;
Sagara, Issaka ;
Sidibe, Bakary ;
Toure, Sekou ;
Dembele, Demba ;
Dama, Souleymane ;
Ouologuem, Dinkorma ;
Dicko, Alassane ;
Doumbo, Ogobara K. .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2008, 78 (03) :455-461