Use of MALDI-TOF MS (Bruker Daltonics) for identification of Mycobacterium species isolated directly from liquid medium

被引：13

作者：

Fernandez-Esgueva, Marta ^{[1
]}

Fernandez-Simon, Rebeca ^{[1
]}

Luisa Monforte-Cirac, Maria ^{[1
]}

Isabel Lopez-Calleja, Ana ^{[1
]}

Fortuno, Blanca ^{[1
]}

Vinuelas-Bayon, Jesus ^{[1
,2
]}

机构：

[1] Hosp Univ Miguel Servet IIS Aragon, Serv Microbiol, Zaragoza, Spain

[2] Soc Espanola Microbiol & Enfermedades Infecciosas, Grp Estudio Infecc Micobacterias GEIM, Madrid, Spain

来源：

ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA | 2021年 / 39卷 / 05期

关键词：

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS); Mycobacteria; MGIT liquid medium; DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY;

D O I：

10.1016/j.eimc.2020.05.011

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: The aim of this study was to describe the evaluation of the use of MALDI-TOF MS for the identification of non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis directly from liquid MGIT cultures from January 2017 to December 2017. Material/methods: A total of 155 isolates (mainly respiratory) were analyzed by MALDI-TOF MS (Bruker Daltonics) directly from MGIT liquid medium with a previous extraction procedure. Results: MALDI-TOF MS generated acceptable scores for 152 isolates (98.06%). Fifty isolates were identified as M. tuberculosis complex and the remaining 105 as NTM (M. abscessus subsp. abscessus, M. avium, M. celatum, M chelonae, M. chimaera, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. lentiflavum, M. mageritense, M. mucogenicum and M. xenopi). Conclusions: These results indicate that MALDI-TOF MS can be useful to identify mycobacteria directly from MGIT cultures and is an accurate, rapid and cost-effective system to be used as a routine method. (C) 2020 Elsevier Espana, S.L.U. and Sociedad Espanola de Enfermedades Infecciosas y Microbiologia Clinica. All rights reserved.

引用

页码：241 / 243

页数：3

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