Dual-quenching NBD-based fluorescent probes for separate detection of H2S and Cys/Hcy in living cells

被引：18
作者：
Jiang, Yaqing [1 ]
Ji, Xiuru [2 ]
Zhang, Changyu [1 ]
Xi, Zhen [3 ,4 ]
Sun, Lu [2 ]
Yi, Long [1 ,3 ,4 ]
机构：
[1] Beijing Univ Chem Technol, State Key Lab Organ Inorgan Composites, Beijing 100029, Peoples R China
[2] Tianjin Med Univ, Sch Pharm, Tianjin Key Lab Technol Enabling Dev Clin Therape, Tianjin 300070, Peoples R China
[3] Nankai Univ, State Key Lab Elemento Organ Chem, Tianjin 300071, Peoples R China
[4] Nankai Univ, Dept Chem Biol, Natl Pesticide Engn Res Ctr Tianjin, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2019年 / 17卷 / 36期
关键词：
HYDROGEN-SULFIDE; SENSITIVE DETECTION; RAPID DETECTION; CANCER-CELLS; ON PROBE; GLUTATHIONE; CYSTEINE/HOMOCYSTEINE; DISCRIMINATION; HOMOCYSTEINE; CYSTEINE;
D O I：
10.1039/c9ob01535c
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Biothiols are implicated in different physiological and pathological processes, while abnormal levels of biothiols are related to numerous diseases. It is essential to discriminatively detect these molecules and explore their inherent transformation in living biological samples. Herein, dual-reactive and dual-quenching fluorescent probes based on thiolysis of NBD (7-nitro-1,2,3-benzoxadiazole) thioether/ether/amine for separate detection of H2S and Cys/Hcy were rationally designed. Probe 2 was able to sense H2S (lambda(ex/em) = 405/455 nm) and Cys/Hcy (lambda(ex/em) = 470/550 nm) in the presence of GSH in aqueous buffer. Moreover, the probe not only could be applied for visualization of exogenous H2S and Cys in living cells but also successfully showcased the potential to verify the biosynthesis of endogenous H2S from Cys.
引用
收藏
页码：8435 / 8442
页数：8
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