Site-directed mutagenesis of Clostridium perfringens beta-toxin:: expression of wild-type and mutant toxins in Bacillus subtilis

被引：1

作者：

Steinthorsdottir, V ^{[1
]}

Fridriksdottir, V ^{[1
]}

Gunnarsson, E ^{[1
]}

Andresson, OS ^{[1
]}

机构：

[1] Univ Iceland, Inst Expt Pathol, IS-112 Reykjavik, Iceland

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 158卷 / 01期

关键词：

beta-toxin; Clostridium perfringens; mutagenesis; biological activity;

D O I：

10.1111/j.1574-6968.1998.tb12794.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recombinant beta-toxin has been expressed and secreted from Bacillus subtilis. Biological activity was tested in vivo and in vitro. The lethal dose in mice was determined. Hemolysis of rabbit and sheep erythrocytes was tested but no effect was observed. Seven mutant proteins were produced. Targets for mutagenesis were mostly selected on the basis of the similarity between beta-toxin and alpha-toxin from Staphylococcus aureus, a pore-forming toxin. Mutations of two amino acids affected the lethal dose in mice. Both residues have counterparts in the membrane binding region of alpha-toxin. Alteration of the single cysteine residue did not affect protein function, contrary to previous suggestions. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

引用

页码：17 / 23

页数：7

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