Specific Nucleic Acid Detection Using Photophysical Properties of Quantum Dot Probes

被引：13

作者：

Lim, Sun Hee ^{[1
]}

Buchy, Philippe ^{[2
]}

Mardy, Sek ^{[2
]}

Kang, Moon Sik ^{[1
]}

Yu, Alexey Dan Chin ^{[1
]}

机构：

[1] Inst Pasteur, Songnam 443270, Gyeonggi Do, South Korea

[2] Inst Pasteur, Virol Unit, Phnom Penh, Cambodia

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 03期

关键词：

RESONANCE ENERGY-TRANSFER; HYBRIDIZATION; DNA; SEQUENCES; CLEAVAGE; BINDING; SAMPLES; ASSAY;

D O I：

10.1021/ac902039n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In the present study, we report a novel separation-free method to detect and quantify avian influenza virus A (H5N1) nucleic acid without amplification, based on the alteration of photophysical parameters of quantum dot (QD) probes after hybridization with specific complementary target DNA. The target DNA was quantified in a custom-made portable device by simultaneously measuring lifetime and quenching of the QD probes. QD probes (25-mer) showed a 30% lifetime reduction and 40% fluorescence quenching when hybridized with complementary 25-mer target DNA. In comparison with a conventional QD-based assay, this assay provides a simple quantitation of nucleic acids with a single labeling step.

引用

页码：886 / 891

页数：6

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