Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G

被引：14

作者：

Gao, Q

Piret, JM

Adrio, JL

Demain, AL ^{[1
]}

机构：

[1] Drew Univ, Charles A Dana Res Inst Sci Emeriti, Madison, NJ 07940 USA

[2] MIT, Dept Biol, Cambridge, MA 02139 USA

[3] Northeastern Univ, Dept Biol, Boston, MA 02115 USA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2003年 / 30卷 / 03期

关键词：

antibiotics; bioconversions; deacetoxycephalosporin; expandase; directed evolution; penicillin;

D O I：

10.1007/s10295-003-0034-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We examined the performance of Streptomyces lividans strain W25 containing a hybrid expandase (deacetoxycephalosporin C synthase; DAOCS) gene, obtained by in vivo recombination between the expandase genes of S. clavuligerus and Nocardia lactamdurans for resting-cell bioconversion of penicillin G to deacetoxycephalosporin G. Strain W25 carried out a much more effective level of bioconversion than the previously used strain, S. clavuligerus NP1. The two strains also differed in the concentrations of FeSO4 and a-ketoglutarate giving maximal activity. Whereas NP1 preferred 1.8 mM FeSO4 and 1.3 mM alpha-ketoglutarate, recombinant W25 performed best at 0.45 mM FeSO4 and 1.9 mM alpha-ketoglutarate.

引用

收藏

页码：190 / 194

页数：5

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