Cloning and characterization of the gene encoding the primary σ-factor of Campylobacter jejuni

被引：0

作者：

Wösten, MMSM ^{[1
]}

Boeve, M ^{[1
]}

Gaastra, W ^{[1
]}

van der Zeijst, B ^{[1
]}

机构：

[1] Univ Utrecht, Fac Vet Med, Dept Bacteriol, Inst Infect Dis & Immunol, NL-3508 TD Utrecht, Netherlands

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 162卷 / 01期

关键词：

Campylobacter jejuni; rpoD; sigma(70);

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The rpoD gene encoding the primary sigma-factor of Campylobacter jejuni was amplified from genomic DNA with degenerate oligonucleotide primers. The complete gene encodes a polypeptide of 622 amino acids and has a deduced M-r of 72.6 kDa. This polypeptide is 40% identical to the RpoD (sigma(70)) protein of Escherichia coli and has 66% identity with the Helicobacter pylori RpoD protein. A C, jejuni sigma(70) promoter, not recognized by the E. coli sigma(70) factor, could be activated in this bacterium in the presence of the cloned C. jejuni RpoD protein. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

引用

页码：97 / 103

页数：7

共 21 条

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