Long-Chain Acyl-CoA Synthetase is Associated with the Growth of Malassezia spp.

被引：11

作者：

Tenagy ^{[1
]}

Tejima, Kengo ^{[1
]}

Chen, Xinyue ^{[1
]}

Iwatani, Shun ^{[1
]}

Kajiwara, Susumu ^{[1
]}

机构：

[1] Tokyo Inst Technol, Sch Life Sci & Technol, Midori Ku, J3-7,4259 Nagatsuta, Yokohama, Kanagawa 2268501, Japan

来源：

JOURNAL OF FUNGI | 2019年 / 5卷 / 04期

关键词：

Malassezia; acyl-CoA synthetase; fatty acid uptake; FATTY-ACID; SEBORRHEIC DERMATITIS; INHIBITION; TRIACSIN; PROTEINS; DANDRUFF; ENZYMES; GLOBOSA; YEAST; SKIN;

D O I：

10.3390/jof5040088

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The lipophilic fungal pathogen Malassezia spp. must acquire long-chain fatty acids (LCFAs) from outside the cell. To clarify the mechanism of LCFA acquisition, we investigated fatty acid uptake by this fungus and identified the long-chain acyl-CoA synthetase (ACS) gene FAA1 in three Malassezia spp.: M. globosa, M. pachydermatis, and M. sympodialis. These FAA1 genes could compensate for the double mutation of FAA1 and FAA4 in Saccharomyces cerevisiae, suggesting that Malassezia Faa1 protein recognizes exogenous LCFAs. MgFaa1p and MpFaa1p utilized a medium-chain fatty acid, lauric acid (C12:0). Interestingly, the ACS inhibitor, triacsin C, affected the activity of the Malassezia Faa1 proteins but not that of S. cerevisiae. Triacsin C also reduced the growth of M. globosa, M. pachydermatis, and M. sympodialis. These results suggest that triacsin C and its derivatives are potential compounds for the development of new anti-Malassezia drugs.

引用

页数：13

共 34 条

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