Potency testing of up to twenty cannabinoids by liquid chromatography diode array detector with optional electrospray ionization time-of-flight mass spectrometry

被引:8
作者
Song, Liguo [1 ]
Valenzuela, Gabrielle [1 ]
Carlson, Shelby [1 ]
Dodson, Zachary [1 ]
Adisa, Mojisola [1 ]
机构
[1] Western Illinois Univ, Dept Chem, Macomb, IL 61455 USA
基金
美国国家科学基金会;
关键词
Cannabinoid; Diode array detector; Hemp; Liquid chromatography; Marijuana; Mass spectrometry; SATIVA L; ACID; THC; CBD;
D O I
10.1016/j.aca.2022.339827
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The growing popularity of Cannabis sativa L. and its widespread use for medical and recreational purposes have created an urgent demand of accurate and reliable analytical methods to identify and quantify a growing number of cannabinoids. To meet this demand, a liquid chromatography diode array detector (LC-DAD) method has been developed, validated, and applied in analysis of cannabinoids in nine samples of plant materials of marijuana, six samples of marijuana cigarettes, five samples of hemp flowers, one sample of hemp cigarette, and two samples of A8-tetrahydrocannabinol (A8-THC) fortified hemp flowers. The method has achieved significant improvements over published methods, which was characterized of 20 targeted cannabinoids, 18 quantified cannabinoids, baseline resolution among quantified cannabinoids, a low limit of quantification (0.02 mu g mL-1), a wide linear range (0.02-25 mu g mL-1 or 0.04-50% (w/w)), a unique experiment to track the recovery of sample preparation in real time by spiking abnormal cannabidiol (CBD), and a well-assessed specificity by electrospray ionization time-of-flight mass spectrometry (ESI/TOFMS). Precision and accuracy were assessed using quality control (QC) samples at three concentration levels, i.e., 0.02, 0.5, and 12.5 mu g mL-1, in triplicates with inter-day and intraday precision of less than 15% relative standard deviation (RSD) and accuracy of less than +/- 15% relative error, therefore meeting the requirements by the ISO 17025 standards. Additionally, ESI/TOFMS has discovered seven unknown cannabinoids, including one structural isomer of cannabigerol (CBG), one structural isomer of cannabinolic acid (CBNA), four structural isomers of A9-THC, and one structural
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页数:9
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