Tailoring new enzyme functions by rational redesign

被引：85
作者：
Cedrone, F
Ménez, A
Quéméneur, E
机构：
[1] Ctr Etud Saclay, CEA, Dept Ingn & Etud Prot, F-91191 Gif Sur Yvette, France
[2] CEA, Dept Ingn & Etud Prot, SBTN, DE Valrho, F-30207 Bagnols Sur Ceze, France
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2000年 / 10卷 / 04期
关键词：
D O I：
10.1016/S0959-440X(00)00106-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Site-directed mutagenesis is still a very efficient strategy to elaborate improved enzymes. Recently, advances have been made in developing rational strategies aimed at reshaping enzyme specificities and mechanisms, and at engineering biocatalysts through molecular assembling. These knowledge-based studies greatly benefit from the most recent computational analyses of enzyme structures and functions. The combination of rational and combinatorial methods opens up new vistas in the design of stable and efficient enzymes.
引用
收藏
页码：405 / 410
页数：6
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