Two-Step In Vivo Tumor Targeting by Biotin-Conjugated Antibodies and Superparamagnetic Nanoparticles Assessed by Magnetic Resonance Imaging at 1.5 T

被引:16
作者
Baio, Gabriella [1 ]
Fabbi, Marina [2 ]
Salvi, Sandra [3 ]
de Totero, Daniela [2 ]
Truini, Mauro [3 ]
Ferrini, Silvano [2 ]
Neumaier, Carlo Emanuele [1 ]
机构
[1] Natl Canc Inst, Dept Diagnost Imaging, IST, I-16132 Genoa, Italy
[2] Natl Canc Inst, Unit Immunol Therapy, IST, I-16132 Genoa, Italy
[3] Natl Canc Inst, Dept Pathol, IST, I-16132 Genoa, Italy
关键词
Magnetic resonance imaging; Iron oxide particles; In vivo small animal MRI; Targeted contrast agent; Antibody; CELLS; CONTRAST; MR; EXPRESSION; TRACKING; BRAIN; VITRO;
D O I
10.1007/s11307-009-0264-6
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
The purpose of this study was to assess two-step in vivo tumor targeting by specific biotin-conjugated antibodies and ultrasmall superparamagnetic iron oxide (USPIO)-anti-biotin nanoparticles as contrast agents for magnetic resonance imaging (MRI) at 1.5 T. D430B human lymphoma cells, expressing the CD70 surface antigen, were injected either s.c. or i.v. to induce pseudo-metastases in NOD/SCID mice. Thirty micrograms of biotin-conjugated monoclonal anti-CD70 was injected i.v., followed 4 h later by 8 A mu mol Fe/Kg USPIO-anti-biotin. After 24 h, MRI was performed on T2* and b-FFE sequences. Signal intensity (SI) was calculated before and after USPIO-anti-biotin administration. Subcutaneous xenografts showed a dishomogeneous 30% decrease in SI on T2* with anti-CD70 + USPIO-anti-biotin treatment. Pseudo-metastatic xenografts showed a slight reduction in SI on T2*, but a 60% decrease in SI on b-FFE-weighted sequences. Prussian blue staining confirmed the presence of iron nanoparticles in the excised tumors. MRI at 1.5 T can detect tumors by a two-step in vivo biotin-based protocol, which may allow the targeting of any cell surface antigen.
引用
收藏
页码:305 / 315
页数:11
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