Structural basis of mitochondrial transcription

被引：74

作者：

Hillen, Hauke S. ^{[1
]}

Temiakov, Dmitry ^{[2
,3
]}

Cramer, Patrick ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Mol Biol, Gottingen, Germany

[2] Rowan Univ, Sch Osteopath Med, Dept Cell Biol, Stratford, NJ USA

[3] Thomas Jefferson Univ, Dept Biochem & Mol Biol, Kimmel Canc Ctr, Philadelphia, PA 19107 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2018年 / 25卷 / 09期

基金：

欧洲研究理事会;

关键词：

DNA-BINDING PROTEIN; RNA-POLYMERASE; YEAST MITOCHONDRIAL; RIBOSOMAL-RNA; CRYSTAL-STRUCTURE; IN-VIVO; METHYLTRANSFERASE ACTIVITY; PROMOTER RECOGNITION; INITIATION COMPLEX; HEAVY-STRAND;

D O I：

10.1038/s41594-018-0122-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mitochondrial genome is transcribed by a single-subunit DNA-dependent RNA polymerase (mtRNAP) and its auxiliary factors. Structural studies have elucidated how mtRNAP cooperates with its dedicated transcription factors to direct RNA synthesis: initiation factors TFAM and TFB2M assist in promoter-DNA binding and opening by mtRNAP while the elongation factor TEFM increases polymerase processivity to the levels required for synthesis of long polycistronic mtRNA transcripts. Here, we review the emerging body of structural and functional studies of human mitochondrial transcription, provide a molecular movie that can be used for teaching purposes and discuss the open questions to guide future directions of investigation.

引用

页码：754 / 765

页数：12

共 129 条

[1] Replication-transcription switch in human mitochondria [J].

Agaronyan, Karen ;

Morozov, Yaroslav I. ;

Anikin, Michael ;

Temiakov, Dmitry .

SCIENCE, 2015, 347 (6221) :548-551

[2] Human mitochondrial DNA is packaged with TFAM [J].

Alam, TI ;

Kanki, T ;

Muta, T ;

Ukaji, K ;

Abe, Y ;

Nakayama, H ;

Takio, K ;

Hamasaki, N ;

Kang, DC .

NUCLEIC ACIDS RESEARCH, 2003, 31 (06) :1640-1645

[3] EXPRESSION OF MITOCHONDRIAL GEUOME IN HELA CELLS .7. SYMMETRICAL IN-VIVO TRANSCRIPTION OF MITOCHONDRIAL DNA IN HELA CELLS [J].

ALONI, Y ;

ATTARDI, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (08) :1757-&

[4] EXPRESSION OF MITOCHONDRIAL GENOME IN HELA CELLS .2. EVIDENCE FOR COMPLETE TRANSCRIPTION OF MITOCHONDRIAL DNA [J].

ALONI, Y ;

ATTARDI, G .

JOURNAL OF MOLECULAR BIOLOGY, 1971, 55 (02) :251-&

[5] The human mitochondrial transcription termination factor (mTERF) is fully active in vitro in the non-phosphorylated form [J].

Asin-Cayuela, J ;

Schwend, T ;

Farge, G ;

Gustafsson, CM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (27) :25499-25505

[6] Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp [J].

Bernecky, Carrie ;

Plitzko, Juergen M. ;

Cramer, Patrick .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2017, 24 (10) :809-+

[7] Strikingly Bacteria-Like and Gene-Rich Mitochondrial Genomes throughout Jakobid Protists [J].

Burger, Gertraud ;

Gray, Michael W. ;

Forget, Lise ;

Lang, B. Franz .

GENOME BIOLOGY AND EVOLUTION, 2013, 5 (02) :418-438

[8] MTERF4 Regulates Translation by Targeting the Methyltransferase NSUN4 to the Mammalian Mitochondrial Ribosome [J].

Camara, Yolanda ;

Asin-Cayuela, Jorge ;

Park, Chan Bae ;

Metodiev, Metodi B. ;

Shi, Yonghong ;

Ruzzenente, Benedetta ;

Kukat, Christian ;

Habermann, Bianca ;

Wibom, Rolf ;

Hultenby, Kjell ;

Franz, Thomas ;

Erdjument-Bromage, Hediye ;

Tempst, Paul ;

Hallberg, B. Martin ;

Gustafsson, Claes M. ;

Larsson, Nils-Goran .

CELL METABOLISM, 2011, 13 (05) :527-539

[9] On the evolution of the single-subunit RNA polymerases [J].

Cermakian, N ;

Ikeda, TM ;

Miramontes, P ;

Lang, BF ;

Gray, MW ;

Cedergren, R .

JOURNAL OF MOLECULAR EVOLUTION, 1997, 45 (06) :671-681

[10] Sequences homologous to yeast mitochondrial and bacteriophage T3 and T7 RNA polymerases are widespread throughout the eukaryotic lineage [J].

Cermakian, N ;

Ikeda, TM ;

Cedergren, R ;

Gray, MW .

NUCLEIC ACIDS RESEARCH, 1996, 24 (04) :648-654

← 1 2 3 4 5 6 7 8 9 10 →