Utilization of waste dried Mangifera indica leaves for extraction of mangiferin by conventional batch extraction and advance three-phase partitioning

Waste dried Mangifera indica (mango) leaves were utilized as a promising source of mangiferin, a valuable biomolecule. Different extraction procedures like three-phase partitioning and batch extraction were evaluated to get an optimum yield of mangiferin. Various parameters affecting the extraction process like salts, time, slurry to t-butanol ratio, ammonium sulfate, pH 6 and solute to solvent ratio were optimized. In three-phase partitioning, the yield of mangiferin obtained was 28 mg/g at a concentration of 40% w/v ammonium sulfate, pH 6, slurry to t-butanol ratio 1:1, solute to solvent ratio 1:40 and time 2 h. Conventional batch extraction using water as solvent resulted in a yield of 23 mg/g in 2 h 40 min, while Soxhlet extraction, as a reference method, yielded 57 mg/g in 5 h. Thus, conventional batch extraction with water can be used for large-scale operations of mangiferin extraction.