The Expression Analysis of Periostin in Human Breast Cancer

被引：45
作者：
Zhang, Yanan [1 ]
Zhang, Guangyuan [1 ]
Li, Junsheng [1 ]
Tao, Qingsong [1 ]
Tang, Wenhao [1 ]
机构：
[1] Southeast Univ, Dept Gen Surg, Affiliated Zhong Da Hosp, Nanjing 210009, Peoples R China
来源：
JOURNAL OF SURGICAL RESEARCH | 2010年 / 160卷 / 01期
关键词：
breast cancer; periostin; cell growth; oncogene; ANCHORAGE-INDEPENDENT GROWTH; CELL-SURVIVAL; INTEGRINS; ADHESION; MICROENVIRONMENT; INVASIVENESS; INVASION; PROTEIN; PATHWAY; LIGAND;
D O I：
10.1016/j.jss.2008.12.042
中图分类号：
R61 [外科手术学];
学科分类号：
摘要：
Background. To investigate the expression of periostin in breast cancer and its clinical relevance. Methods. Periostin mRNA expression in breast tissues was measured by RT-PCR. Periostin protein in breast tissues was evaluated by Western blot. Immunohistochemistry method (Envision method) was carried out to detect the expression of periostin in breast cancer tissues of different clinical stages and metastasized lymph nodes, as well as benign breast disease. Results. The expression of periostin in cancer tissue was increased at both mRNA level (P < 0.05) and protein level (P < 0.05) compared with benign and normal breast tissues. The periostin protein expression level increased with breast cancer clinical stages as shown by IHC. Conclusion. Periostin was overexpressed in breast cancer tissues as opposed to normal breast, which implies its role in the pathogenesis and development of this disorder. (C) 2010 Elsevier Inc. All rights reserved.
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页码：102 / 106
页数：5
相关论文
共 20 条
[1]   Periostin potently promotes metastatic growth of colon cancer by augmenting cell survival via the Akt/PKB pathway [J].
Bao, SD ;
Ouyang, G ;
Bai, XF ;
Huang, Z ;
Ma, CY ;
Liu, M ;
Shoo, R ;
Anderson, RM ;
Rich, JN ;
Wang, XF .
CANCER CELL, 2004, 5 (04) :329-339
[2]   Periostin promotes invasiveness and resistance of pancreatic cancer cells to hypoxia-induced cell death:: role of the β4 integrin and the PI3k pathway [J].
Baril, P. ;
Gangeswaran, R. ;
Mahon, P. C. ;
Caulee, K. ;
Kocher, H. M. ;
Harada, T. ;
Zhu, M. ;
Kalthoff, H. ;
Crnogorac-Jurcevic, T. ;
Lemoine, N. R. .
ONCOGENE, 2007, 26 (14) :2082-2094
[3]   Periostin creates a tumor-supportive microenvironment in the pancreas by sustaining fibrogenic stellate cell activity [J].
Erkan, Mert ;
Kleeff, Joerg ;
Gorbachevski, Andre ;
Reiser, Carolin ;
Mitkus, Tomas ;
Esposito, Irene ;
Giese, Thomas ;
Buechler, Markus W. ;
Giese, Nathalia A. ;
Friess, Helmut .
GASTROENTEROLOGY, 2007, 132 (04) :1447-1464
[4]  
Gillan L, 2002, CANCER RES, V62, P5358
[5]   A matter of life and death [J].
Green, DR ;
Evan, GI .
CANCER CELL, 2002, 1 (01) :19-30
[6]   Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor β [J].
Horiuchi, K ;
Amizuka, N ;
Takeshita, S ;
Takamatsu, H ;
Katsuura, M ;
Ozawa, H ;
Toyama, Y ;
Bonewald, LF ;
Kudo, A .
JOURNAL OF BONE AND MINERAL RESEARCH, 1999, 14 (07) :1239-1249
[7]   Taking the study of cancer cell survival to a new dimension [J].
Jacks, T ;
Weinberg, RA .
CELL, 2002, 111 (07) :923-925
[8]   Periostin is down-regulated in high grade human bladder cancers and suppresses in vitro cell invasiveness and in vivo metastasis of cancer cells [J].
Kim, CJ ;
Yoshioka, N ;
Tambe, Y ;
Kushima, R ;
Okada, Y ;
Inoue, H .
INTERNATIONAL JOURNAL OF CANCER, 2005, 117 (01) :51-58
[9]   Periostin promotes invasion and anchorage-independent growth in the metastatic process of head and neck cancer [J].
Kudo, Yasusei ;
Ogawa, Ikuko ;
Kitajima, Shojiro ;
Kitagawa, Masae ;
Kawai, Hidehiko ;
Gaffney, Patrick M. ;
Miyauchi, Mutsumi ;
Takata, Takashi .
CANCER RESEARCH, 2006, 66 (14) :6928-6935
[10]   Vascular injury induces expression of periostin - Implications for vascular cell differentiation and migration [J].
Lindner, V ;
Wang, QZ ;
Conley, BA ;
Friesel, RE ;
Vary, CPH .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2005, 25 (01) :77-83
12