DETERMINATION OF FREE URINARY CORTISOL, IN CUSHING'S SYNDROME USING REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

被引：0

作者：

Sugawara, Eduardo Kinio ^{[1
]}

Ribeiro Neto, Luciane Maria ^{[1
]}

do Nascimento Verreschi, Ieda Terezinha ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Dept Med, BR-04039032 Sao Paulo, Brazil

来源：

QUIMICA NOVA | 2010年 / 33卷 / 02期

关键词：

HPLC; cortisol; urine; TANDEM MASS-SPECTROMETRY; ULTRAVIOLET ABSORBENCY DETECTION; STABLE-ISOTOPE DILUTION; CYP3A ACTIVITY; 6-BETA-HYDROXYCORTISOL; PLASMA; HPLC; CORTICOSTEROIDS; PREDNISOLONE;

D O I：

10.1590/S0100-40422010000200036

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Determination of free urinary cord sol is a test of choice in the diagnosis Of Cushing's syndrome. In fit is study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide its internal standard (IS). A BDS-Hypersil-C18(R) column. water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging front 2.50 to 150 mu g/L. and can be used in substitution to the radioimmunoassay technique within this concentration range.

引用

页码：447 / 450

页数：4

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