Screening and detection of apoptosis

被引:181
作者
Huerta, Sergio [1 ]
Goulet, Emily J. [1 ]
Huerta-Yepez, Sara [1 ]
Livingston, Edward H. [1 ]
机构
[1] Univ Texas, SW Med Ctr, VA N Texas Hlth Care Syst, Dept Gastrointestinal & Endocrine Surg, Dallas, TX 75230 USA
关键词
caspases; Bcl-2; Apaf-1; death receptor pathway; AIF; proteosome;
D O I
10.1016/j.jss.2006.07.034
中图分类号
R61 [外科手术学];
学科分类号
摘要
Since programmed cell death was first described by the electron microscopic cellular changes demonstrating an organized form of cell death over 30 years ago, it has undergone a great deal of scrutiny as a potential target for several diseases including cancer. The techniques for the study of apoptosis have evolved accordingly. Methodologies for the study of apoptosis were examined by a MEDLINE search of the English-language literature and are summarized in this review. This review discusses the various ways to study apoptosis with specific assays, reagents, and molecules. The particular advantages and disadvantages of each method are reviewed. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:143 / 156
页数:14
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