Induction of osteopontin gene expression during mammary gland involution and effects of glucocorticoid on its expression in mammary epithelial cells

被引：6

作者：

Lee, M

Na, S

Jeon, D

Kim, H

Choi, Y

Baik, M ^{[1
]}

机构：

[1] Chonnam Natl Univ, Coll Agr, Biotechnol Res Inst, Dept Genet Engn, Kwangju 500757, South Korea

[2] Seoul Natl Univ, Sch Agr Biotechnol, Suweon 441744, South Korea

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 10期

关键词：

osteopontin gene; involution; mouse mammary gland; dexamethasone;

D O I：

10.1271/bbb.64.2225

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To understand the molecular mechanisms of mammary gland involution, an involution-induced clone was identified from a cDNA library of mouse mammary gland by differential screening. Characterization of a clone by sequencing and northern analysis showed that expression of the osteopontin gene was induced during involution of mouse mammary gland. But induction of the osteopontin gene was not observed in apoptotic HC11 mammary epithelial cells under serum starvation. In HCII cells, dexamethasone treatment from the seeding stage showed five-fold induction of osteopontin gene expression, but the expression was not changed when dexamethasone was added to confluent cells.

引用

页码：2225 / 2228

页数：4