Separation of rhubarb anthraquinones by capillary electrochromatography

被引：16
作者：
Ding, J
Ning, B
Fu, G
Lu, Y
Dong, S [1 ]
机构：
[1] Beijing Med Univ, Sch Pharmaceut Sci, Dept Pharm, Beijing 100083, Peoples R China
[2] Natl Inst Control Pharmaceut & Biol Prod, Div Chem Drugs, Beijing 100050, Peoples R China
来源：
CHROMATOGRAPHIA | 2000年 / 52卷 / 5-6期
关键词：
capillary electrochromatography; packing capillary electrochromatography columns; rhubarb anthraquinones;
D O I：
10.1007/BF02491018
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid, simple method for packing capillary electrochromatography (CEC) columns with HPLC stationary phases is described. The basis of the method is the use of a vacuum to suck a slurry of stationary phase into the fused-silica tubing, a procedure which takes approximately ten seconds only then compression of the stationary phase by means of an HPLC pump. These packed CEC columns have been investigated for the separation of five anthraquinones from rhubarb. Separation of the anthraquinones in Rheum palmatum L. under optimized conditions is presented.
引用
收藏
页码：285 / 288
页数：4
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